中国药房2012，Vol.23Issue(17)：1568-1571,4.DOI:10.6039/j.issn.1001-0408.2012.17.11
促红细胞生成素对局灶性脑缺血再灌注模型大鼠脑组织的保护作用研究
Protective Effect of Erythropoietin on Cerebral Tissue of Rats with Focal Cerebral Ischemia-reperfusion
赵德福 1宁显忠 1常钦达 1杨冬辉1
作者信息
- 1. 辽宁医学院附属第三医院,辽宁锦州121000
- 折叠
摘要
Abstract
OBJECTIVE: To study the protective effects of erythropoietin (EPO) on cerebral tissue of rats with focal cerebral ischemia-reperfusion. METHODS: SD rats were randomly divided into sham operation group, model group and EPO low-dose, medium-dose and high-dose (1 000 u·kg‐1, 3 000 u·kg‐1,5 000 u·kg‐1) groups. Focal cerebral ischemia-reperfusion model was established by thread occlusion method and given relevant drugs 2 h after ischemia. Before model rats were decollated to collect hippocampus 3, 6, 12, 24, 48 and 72 h after ischemia-reperfusion respectively, the neurological scores of the rats were observed. Expressions of MMP-9 and VEGF in cerebral tissue were observed by immunohistochemical staining and Western blot (n=12). RESULTS: Compared with sham operation group, the neurological scores of model group increased significantly, and the expression of MMP-9 and VEGF of group increased significantly 12 h after reperfusion (P<0.01; compared with model group, the neurological scores and the expression of MMP-9 decreased while the expression of VEGF increased in EPO groups 12 h after reperfusion (P<0.0l) ; compared with EPO low-dose group, the neurological scores and the expression of MMP-9 decreased significantly while the expression of VEGF increased in EPO medium-dose and high-dose groups (P<0.05). CONCLUSION: EPO can protect the extracellular matrix role by decreasing the expression of MMP-9 and enhance the rehabilitation ability of peripheral micrangium by increasing the expression of VEGF to protect cerebral tissue from focal cerebral ischemia-reperfusion injury.关键词
促红细胞生成素/脑缺血再灌注/大鼠/基质金属蛋白酶9/血管内皮生长因子Key words
Erythropoietin/ Cerebral ischemia-reperfusion/ Rats/ MMP-9/ VEGF
分类
医药卫生引用本文复制引用
赵德福,宁显忠,常钦达,杨冬辉..促红细胞生成素对局灶性脑缺血再灌注模型大鼠脑组织的保护作用研究[J].中国药房,2012,23(17):1568-1571,4.
