中国药房2012，Vol.23Issue(17)：1571-1573,3.DOI:10.6039/j.issn.1001-0408.2012.17.12
曲美他嗪对庆大霉素致大鼠肾毒性的预防作用研究
Study on Preventative Effects of Trimetazidine on Gentamicin-induced Nephrotoxicity in Rats
伊娜 1刘敏 2李忠东 2张福成2
作者信息
- 1. 河北北方学院,河北张家口075000
- 2. 空军总医院药学部,北京100142
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the preventative effects of trimetazidine on gentamicin (GM)-induced nephrotoxicity in rats. METHODS: The rats were divided into solvent control group, trimetazidine low-dose and high-dose groups (5, 10 mg·kg‐1· d‐1), gentamicin group (100 mg·kg‐1·d‐1), trimetazidine (5 mg‐·kg‐1·d‐1)+gentamicin (100 mg·kg‐1·d‐1) group and trimetazidine (10 mg·kg‐1·d‐1)+gentamicin (100 mg·kg‐1·d‐1) with 6 rats in each group. Trimetazidine was administered intragastncally for 2 days; from the third day, gentamicin was given intraperitoneally 30 min after intragastric administration for consecutive 7 days. Other groups were given normal saline accordingly. Rats were sacrificed 24 h after the last administration, and the concentration of gentamicin in renal tissue of rats was determined by fluorescence polarization immunoassay. The apoptotic renal tubular epithelial cells were examined by TUNEL method. Morphologic changes of kidney were determined by HE staining. RESULTS: Compared with gentamicin group, the concentrations of gentamicin in renal tissue of trimetazidine (10 mg·kg‐1·d‐1)+gentamicin group decreased significantly (P<0.05); the numbers of apoptotic renal tubular epithelial cells in trimetazidine (5 mg‐·kg'-d^+gentami-cin group and trimetazidine (10 mg‐·kg‐1·d‐1)+gentamicin group decreased significantly (P<0.05 or P<0.01). The inflammatory cell infiltration and pathology were found in renal tissue of rats in gentamicin group. Renal pathology of renal tissue was improved in trimetazidine (5 mg·kg‐1·d‐1)+gentamicin group and trimetazidine (10 mg·kg‐1·d‐1)+gentaniicin group. CONCLUSION: Trimetazidine can protect the rat against gentamicin-induced nephrotoxicity by decreasing the accumulation of gentamicin in kidney.关键词
曲美他嗪/庆大霉素/大鼠/细胞凋亡/肾毒性/病理变化/预防作用Key words
Trimetazidine / Gentamicin/ Rats/ Apoptosis/ Nephrotoxicity/ Pathology change/ Preventative effect
分类
医药卫生引用本文复制引用
伊娜,刘敏,李忠东,张福成..曲美他嗪对庆大霉素致大鼠肾毒性的预防作用研究[J].中国药房,2012,23(17):1571-1573,3.
