中国药房2012，Vol.23Issue(17)：1580-1582,3.DOI:10.6039/j.issn.1001-0408.2012.17.15
HPLC-荧光法测定莫西沙星在犬体内的血药浓度及其药动学研究
Determination of Plasma Concentration of Moxifloxacin in Beagle Dogs by HPLC-fluorescent Method and Its Pharmacokinetic Study
徐建平 1易红 2袁浩宇2
作者信息
- 1. 都江堰市人民医院,四川都江堰611830
- 2. 成都核工业四一六医院,成都610051
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for determining the plasma concentration of moxifloxacin in Beagle dogs, and to study pharmacokinetic characteristics of it. METHODS: 6 Beagle dogs were given a single dose of moxifloxacin 120 mg. The plasma concentration of moxifloxacin in samples within 72 h after administration was determined by HPLC-fluorescent method and pharmacokinetic parameters was calculated by using DAS software. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of acetonitrile-1.0 mol·L‐1 acetic acid solution (25:75, pH=2.0) at the flow rate of 1.0 mL·min‐1. The excitation wavelength was set at 273 nm and emission wavelength was set at 488 run. RESULTS: The liner range of moxifloxacin was 0.1~20.0 μg·mL‐1 (r=0.999 2) with relative recovery rate of 89.5%~96.7%. The RSDs of intra-day and inter-day were less than 6%. The pharmacokinetics of moxifoxacin was fitted to two-compartment open pharmacokinetic model. The main pharmacokinetic parameters of moxifloxacin were as follows: Cmax: (10.25 ±4.21) μg·mL‐1, tmax: (3.0± 1.0) h, t1/2β: (9.76±2.48) h, AUCo~24h: (142.10 ± 49.80) mg·h-L‐1. CONCLUSION: The method is accurate, sensitive and rapid, and suitable for plasma concentration determination and pharmacokinetic study of moxifloxacin.关键词
莫西沙星/高效液相色谱-荧光法/犬/血药浓度/药动学Key words
Moxifloxacin/ HPLC-fluorescent method/ Beagle dogs/ Plasma concentration/ Pharmcokinetics
分类
医药卫生引用本文复制引用
徐建平,易红,袁浩宇..HPLC-荧光法测定莫西沙星在犬体内的血药浓度及其药动学研究[J].中国药房,2012,23(17):1580-1582,3.
