中国药房2012，Vol.23Issue(17)：1613-1615,3.DOI:10.6039/j.issn.1001-0408.2012.17.27
RP-HPLC法同时测定利明滴眼液中维生素C和维生素B1的含量
Simultaneous Determination of Vitamin C and Vitamin B1 in Liming Eye Drops by RP-HPLC
何笑荣 1姜文清 1霍秀颖 1崔嘉芯 2邹定1
作者信息
- 1. 卫生部北京医院药学部,北京100730
- 2. 温州医学院,浙江温州 325035
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the simultaneous determination of vitamin C and vitamin B1 in Liming eye drops. METHODS:RP-HPLC method was adopted. The separation was carried out on Alltim Cu column with mobile phase consisted of methanol-30 mmol·L‐1 ammonium dihydrogen phosphate solution (5:95, adjusted pH to 3.2) at the flow rate of 0.6 mL· min‐1. The detection wavelength was set at 246 nm and column temperature was 25 ℃. RESULTS:The linear ranges of vitamin C and vitamin B, were 7.5~360 μg·mL‐1 (r=0.999 6, n=5), 11.92-198.64 μg·mL‐1(r=0.999 8, n=5),respectively. The detection limits were 7.5 ng and 11.9 ng,respectively. The RSDs of intra-day and inter-day and reproducibility did not exceed 2.0%,respectively. The average recoveries were 102.85% and 101.43% (n=3). CONCLUSION: The method is simple, sensitive, accurate and could be applied to the quality control of Liming eye drops.关键词
反相高效液相色谱法/利明滴眼液/维生素C/维生素B1/含量测定Key words
RP-HPLC/ Liming eye drops/ Vitamin C/ Vitamin B1/ Content determination
分类
医药卫生引用本文复制引用
何笑荣,姜文清,霍秀颖,崔嘉芯,邹定..RP-HPLC法同时测定利明滴眼液中维生素C和维生素B1的含量[J].中国药房,2012,23(17):1613-1615,3.
