中国药房2012，Vol.23Issue(23)：2127-2129,3.DOI:10.6039/j.issn.1001-0408.2012.23.06
注射用羟基红花黄色素A对大鼠脑神经元线粒体的保护作用研究
Protective Effect of HSYA Freeze-dried Powder on Brain Neuronal Mitochondria of Rats
徐露 1董志1
作者信息
- 1. 重庆医科大学重庆市生化与分子药理重点实验室,重庆400016
- 折叠
摘要
Abstract
OBJECTIVE: To study the protective effect of HSYA freeze-dried powder on brain neuronal mitochondria of rats. METHODS: Cerebral ischemia-reperfusion model was induced by suture method. Model rats were divided into 5 groups, I.e. Sham operation group (constant volume normal saline), model group (constant volume normal saline), nimodipine group(1.0 mg·kg-1), HSYA high-dose, medium-dose and low-dose groups (10.24 mg·kg-1,5.12 mg·kg-1,2.56 mg·kg-1). They were given relevant drugs intravenously via tail once a day for consecutive 3 days. The content of PAF in serum and the activity of Na+-K+-ATP enzyme, Ca2+-ATP enzyme and Mg2+-ATP enzyeme in hippocampus were all determined. We observed ultrastructure of mitochondrial in gray area with electron microscope. RESULTS: Compared with model group, the content of PAF in serum of HSYA high-dose, medium-dose and low-dose groups could decrease significantly, and the activity of Na+-K+-ATP enzyme, Ca2+-ATP enzyme and Mg2+-ATP enzyme in hippocampus could increase significantly(P<0.01). Mitochondrial structure was more complete and mitochondrial damaging in HSYA groups was slighter than that in model group. CONCLUSION: HSYA freeze-dried powder has a significant protective effect on brain mitochondria.关键词
羟基红花黄色素A/线粒体/血小板活化因子Key words
HSYA/ Mitochondria /PAF分类
医药卫生引用本文复制引用
徐露,董志..注射用羟基红花黄色素A对大鼠脑神经元线粒体的保护作用研究[J].中国药房,2012,23(23):2127-2129,3.
