中国医科大学学报2012，Vol.41Issue(5)：388-390,3.
A型激酶锚定蛋白γ在小鼠卵母细胞减数分裂过程中的表达
Expression and Modification of AKAP7γ during Meiosis in Mouse Oocytes
摘要
Abstract
Objective In the mammalian oocytes,protein kinase A (PKA) has critical functions in the maintenance of meiotic arrest and oocyte maturation. Spatial regulation of PKA is accomplished by its sequestration via A-kinase anchor proteins (AKAPs). In this study,we aimed to examine the endogenous expression of AKAP7γ in the germinal vesicle (CV) and MII stage of mouse oocytes. Methods The CV and MII stage of mouse oocytes for the presence and modification of the AKAP7γwere examined by Western blot. Results AKAP7γ exhibited a retarded electrophoreoc motility at MII of meiosis compared with CV stage in mouse oocytes. The motility shift of AKAP7γ was susceptible to okadaic acid and alkaline phosphatase. Conclusion Mouse oocytes at MII of meiosis express a phosphorylated form of AKAP7γ. AKAPγ might function in mouse oocytes not only as an anchoring protein but also as a substrate and effector for the anchored kinases.关键词
卵母细胞/A型激酶锚定蛋白γ/磷酸化Key words
oocyle/ AKAP7γ/ phosphorylation分类
生物科学引用本文复制引用
王述森,杨雪鹰,罗阳,张阳..A型激酶锚定蛋白γ在小鼠卵母细胞减数分裂过程中的表达[J].中国医科大学学报,2012,41(5):388-390,3.基金项目
国家自然科学基金青年基金资助项目(30800392) （30800392）
辽宁省高校科研计划(2008807) （2008807）
