中国药理学通报2012，Vol.28Issue(8)：1079-1083,5.DOI:10.3969/j.issn.1001-1978.2012.08.010
细胞亲和-UPLC-QTOF测定蟾蜍甾烯与宫颈癌HeLa细胞的亲和及与化合物计算分子属性的相关性分析
Cell extraction-UPLC-QTOF determination of the biological affinity of bufadienolides with HeLa cells and their correlation with properties computed from structure
摘要
Abstract
Aim To assay the affinity rate of bufadien-olides with HeLa cell line, and to analyze the correlation between the bioaffinity rates and the properties computed from structure of bufadienolides. Method The chloroform extract of Chansu was added into cultured HeLa cells. The contents of bufadienolides in the supernatant and cell were measured UPLC-QTOF. 10 properties computed from structure of bufadienolides were collected from Pubchem database. Result The coefficient of correlation between XLogP3 , H-Bond Donor and Topological Polar Surface Area with the de- crease of bufadienolides in cell supernatant after affini-ty, was r = 0. 865 ( P < 0. 01) , r = - 0. 901 ( P < 0. 01) , r = -0. 657 (P < 0. 05) , respectively. Other computed properties had no significant correlation with the bioaffinity rates. Conclusion Bufadienolides have different levels of affinity with HeLa cells, which correlates with their properties computed from structure.关键词
蟾蜍甾烯/HeLa/非特异性细胞亲和/超高效液相-电喷雾飞行时间质谱/计算分子属性/脂水分配参数/氢键供体/相关性分析Key words
bufadienolides/ HeLa cells/ non-specific cell extraction/ UPLC-QTOF/ properties computed from structure/ H-bond donor/ correlation analysis分类
医药卫生引用本文复制引用
周婧,王剑,马宏跃,丁安伟,尚尔鑫,詹瑧,钱大玮,唐于平..细胞亲和-UPLC-QTOF测定蟾蜍甾烯与宫颈癌HeLa细胞的亲和及与化合物计算分子属性的相关性分析[J].中国药理学通报,2012,28(8):1079-1083,5.基金项目
国家自然基金青年基金资助项目(No 81102762,30901894) （No 81102762,30901894）
国家科技支撑计划(No 2011BAI04B04) （No 2011BAI04B04）
教育部新教师基金资助项目(No 20093237120013) （No 20093237120013）
霍英东教育基金会第十二届高等院校青年教师基金资助项目(No 121044),江苏省中医药局项目(No LZ09017) （No 121044）
南京中医药大学青年自然科学基金资助项目(No 09XZR21) （No 09XZR21）
