中国药理学通报2012，Vol.28Issue(8)：1084-1087,4.DOI:10.3969/j.issn.1001-1978.2012.08.011
ERK1/2和Akt通路对控制性低压后处理兔缺血/再灌注损伤脊髓线粒体功能的影响
Effect of Akt and ERK1/2 pathway in the mitochondrial function by controlled low perfusion pressure against spinal ischemia/reperfusion in rabbits
摘要
Abstract
Aim To observe the influence of Akt and ERK1/2 pathway on the mitochondrial function by controlled low perfusion pressure against spinal ischemia-reperfusion. Methods Thirty Japanese white rabbits weighing 2. 0 ~ 2. 5 kg were randomly divided into five groups ( n = 6 ) : sham group, ischemia/reperfusion ( I/ R) group, controlled low perfusion pressure ischemic postconditioning(Post) group, Post + PD98059 (Post + PD) group and Post + LY294002 ( Post + LY) group. Except sham group, the spinal ischemia was induced by occlusion of the infrarenal aorta for 25 min. Post group: postconditioning was accomplished by the blood flow partially restored and the mean blood pressure of the distal aorta was controlled between 5. 99 and 7. 32 kPa by adjusting the volume of the balloon during the first 10 min of reperfusion. Post + PD and Post + LY group: PD98059 ( 3 μg, 20 μl, a specific ERK1/2 inhibitor) and LY294002 (10 μg, 20 μl, a specific PI3K/Akt inhibitor) were intravenously administered 1 minute before ischemic postconditioning. All rabbits were sacrificed 1 d after reperfusion , the spinal cord was removed for determination of the expression of Caspase-3 and Cytochrome C by Western blot and the ultrastructural change of mitochondrial was observed by electronmicroscope. Results Compared with I/R group, the expressions of Caspase-3 and Cytochrome C were decreased, and ultrastructure of mitochondrial was less damaged in Post group. However, the protective effect of Post was reduced by PD98059 and LY294002. Conclusion Controlled low perfusion pressure can reduce the spinal cord I/R injury through activiting PI3K/Akt and ERK1/2 pathways and protecting mitochondrial function.关键词
控制性低压/缺血/再灌注损伤/缺血后处理/脊髓/AKT/ERK/线粒体Key words
controlled low perfusion pressure/ I/R injury / ischemic postcondition/ spinal cord/ Akt/ ERK/ mitochondrial分类
医药卫生引用本文复制引用
艾春雨,江晓菁,马虹,王俊科..ERK1/2和Akt通路对控制性低压后处理兔缺血/再灌注损伤脊髓线粒体功能的影响[J].中国药理学通报,2012,28(8):1084-1087,4.基金项目
国家自然科学基金资助项目(No 30740092) （No 30740092）
