中国药理学通报2012，Vol.28Issue(8)：1088-1092,5.DOI:10.3969/j.issn.1001-1978.2012.08.012
二苯乙烯苷对H2O2诱导血管内皮细胞黏附分子表达的影响
Effect of TSG on expression of adhesion molecule induced by H2O2 on vascular endothelial cell
摘要
Abstract
Aim To study the effect of 2,3,5,4' -tetra-hydroxystilbene-2-O-β-D-glucoside on expression of adhesion molecules P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 induced by H2O2 on human umbilical vein endothelial cells ( HUVECs). Methods HUVECs were cultured in vitro. The experiment was divided into four groups: control group, H2 O2 group, positive group, and TSG group. The expression of P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mRNA and protein were respectively detected by re-verse transcriptase polymerase chain reaction ( RT-PCR ) and enzyme-linked immunosorbent assay ( ELISA). Results After endothelial cells treated with 200 μmol · L-1 H2O2 for 24 h, the expression of P-se-lectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mR-NA and protein level was significant higher. After TSG pretreatment endothelial cells for 4h before treated with 200 μmol · L-1 H2O2, results showed that TSG could inhibit the expression of P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mRNA and protein level induced by H2O2 on endothelial cells. Conclusion TSG can inhibit the expression of adhesion molecules induced by H2O2 on human umbilical vein endothelial cells and accordingly slow down atherosclerosis.关键词
二苯乙烯苷/内皮细胞/动脉粥样硬化/P-selectin/E-selectin/ICAM-1/VCAM-1/MCP-1Key words
TSG/ endothelial cells/ atherosclerosis/ P-selectin/ E-selectin/ ICAM-1/ VCAM-1/ MCP-1分类
医药卫生引用本文复制引用
张彩平,杨滢,田英,乔新惠,龙石银,陈志军,田汝芳..二苯乙烯苷对H2O2诱导血管内皮细胞黏附分子表达的影响[J].中国药理学通报,2012,28(8):1088-1092,5.基金项目
国家自然科学基金资助项目(No 30800474) （No 30800474）
湖南省科技厅项目(No 2010SK3037) （No 2010SK3037）
湖南省教育厅重点项目(No 11A104) （No 11A104）
湖南省研究生创新项目(No CX2010B385,CX2011B378) （No CX2010B385,CX2011B378）
