中国药理学通报2012，Vol.28Issue(8)：1125-1130,6.DOI:10.3969/j.issn.1001-1978.2012.08.020
2,6-二异丙基苯酚、人参皂苷Rg-1和氯化锂逆转电休克后嗅球切除抑郁大鼠学习记忆障碍
2,6-Diisopropylphenol, ginsenoside Rg-1 and lithium protects against the impairment of learning-memory induced by electroconvulsive shock in depressed rats
摘要
Abstract
Aim To explore the reverse of 2,6-Diisopropylphenol , ginsenoside Rg-1, lithium against the impairment of learning-memory and the hyperphospho-rylation of protein Tau induced by electroconvulsive shock and the concentration of glutamic acid in the hippocampus in depressed rats, in order to compare the therapeutic effects and mechanisms of three medicines. Methods As the analysis of variance of factorial design set up two intervention factors which were the e-lectroconvulsive shock groups ( two levels: no disposition ; A course of electroconvulsive shock) and the drug intervention groups ( four levels: Microinjection of 2,6-Diisopropylphenol, ginsenoside Rg-1, lithium and Saline) . Sixty-four adult depression model rats whose olfactory bulbs were removed were randomly divided into eight experimental groups (n = 8 , in each group). The Morris water maze test was started in order to evaluate learning-memory. The content of glutamate in the hippocampus of rats was detected by High Performance Liquid Chromatography. The content of protein Tau which includes Tau-5 and p-AT8 Ser202 in the hippocampus of rats was detected by Western blot. Results 2, 6-Diisopropylphenol, ginsenoside Rg-1 and lithium protected against the impairment of learning-memory induced by electroconvulsive shock in depressed rats. The 2,6-Diisopropylphenol and ginsenoside Rg-1 reduced the content of glutamate. These three medicines reduced the hyperphosphorylation of protein Tau in the hippocampus of depressed rats. Conclusions 2,6-Diisopropylphenol, ginsenoside Rg-1 and lithium can protect against the impairment of learning-memory induced by electroconvulsive shock in depressed rats though reducing the hyperphosphorylation of protein Tau. The first two are not content with the glutamate in the hippocampus.关键词
2,6-二异丙基苯酚/人参皂苷Rg-1/氯化锂/电休克/学习记忆/谷氨酸/Tau蛋白/过度磷酸化Key words
2,6-Diisopropylphenol/ ginsenoside Rg-1/ lithium/ electroconvulsive therapy/ learning and memory ability/ glutamic acid/ protein Tau/ hyperphosphorylation分类
医药卫生引用本文复制引用
刘超,闵苏,魏珂,刘东,董军,罗洁,黎平,刘小滨..2,6-二异丙基苯酚、人参皂苷Rg-1和氯化锂逆转电休克后嗅球切除抑郁大鼠学习记忆障碍[J].中国药理学通报,2012,28(8):1125-1130,6.基金项目
国家自然科学基金面上资助项目(No 30972831) （No 30972831）
