中国药理学通报2012，Vol.28Issue(8)：1153-1158,6.DOI:10.3969/j.issn.1001-1978.2012.08.026
芹黄素对体外人大肠癌Lovo细胞生物学活性的影响及机制研究
Experimental research of the effect of apigenin on the biological activity of human colon cancer cell line Lovo and its mechanism in vitro
摘要
Abstract
Aim To investigate the effect of apigenin on the biological activity of human colon cancer cell line Lovo and its possible mechanism in vitro. Methods After Lovo cells were cultured and treated with different concentrations of apigenin for 24 , 48 or 72 h, the morphological and ultrastructural changes were observed through light microscope and transmission electron microscope respectively; cell proliferation was measured by methyl thiazolyl tetrazolium ( MTT) color-imetric assay; cell clonogenicity was detected by colony-forming assay; cell cycle distribution and apoptosis were examined by flow cytometry after cells were stained with PI and Annexin V FITC/PI respectively; the expression of Bcl-2, pro-caspase-3 and cleaved caspase-3 in Lovo cells was detected by Western blot a-nalysis. Results Compared with untreated cells, cells treated with apigenin exhibited a marked proliferation and clone-formation inhibition in a dose-time dependent manner. The half maximal inhibitory concentration (IC50) on cell proliferation was approximately 98. 05 μmol · L when Lovo cells were treated with apigenin for 48 h and 33.91 μimol · L for 72 h. Accompanying with the decreased growth, apigenin treatment could also cause a decreased percentage of cells at G0/ G1 phase, a cell cycle block at G2/M phase and an increased rate of apoptosis. Moreover, apigenin treatment could also dramatically downregulate the expression of Bcl-2, pro-caspase-3 and upregulate the expression of cleaved caspase-3 in Lovo cells. Differences had statistical significance (P < 0. 05). Conclusions Apigenin partially inhibits the proliferation of Lovo cells by blocking cell cycle at G2/M phase, promotes apoptosis by downregulating the expression of Bcl-2, pro-caspase-3 and upregulating the expression of cleaved caspase-3 in Lovo cells in vitro and has potential for further development as a chemotherapeutic a-gent for human colon cancer.关键词
芹黄素/Lovo细胞/增殖/细胞周期/凋亡/Bcl-2/caspase-3Key words
apigenin/ Lovo/ proliferation/ cell cycle/ apoptosis/ Bcl-2/ caspase-3分类
医药卫生引用本文复制引用
王胜奇,赵博欣,韩述岭,李国锋..芹黄素对体外人大肠癌Lovo细胞生物学活性的影响及机制研究[J].中国药理学通报,2012,28(8):1153-1158,6.基金项目
广东省自然科学基金资助项目(No 10151051501000057) （No 10151051501000057）
