中国药理学通报2012，Vol.28Issue(8)：1164-1167,4.DOI:10.3969/j.issn.1001-1978.2012.08.028
大鼠血浆中的新藤黄酸HPLC测定及其药代动力学研究
Determination of Gambogenic acid and its pharmacokinetics in rat plasma by HPLC
摘要
Abstract
Aim To develop a HPLC method for the determination of plasma concentration of Gambogenic acid (GNA) and study the pharmacokinetics of GNA in rat plasma. Methods A HPLC method was established for the determination of GNA in rat plasma using gambogic acid as the internal standard. The plasma concentration of GNA in rats was determined by HPLC after intravenous administration of GNA at a dose of 1, 2 and 4 mg · kg-1 , and the pharmacokinetic parameters were calculated by BABP 3. 0. Results Calibration curve was linear over the range of 0. 07 ~ 18. 00 mg · L-1 . The lower limit of quantification was 0. 07 mg · L-1 . The extraction recoveries were higher than 70% . The intra- and inter-day precision ( RSD% ) was lower than 10%. This validated method was successfully applied to a pharmacokinetic study after iv administration of GNA in rats at a dose of 1, 2 and 4 mg · kg-1. The T2/1was(43.61 ±0. 61) ,(46. 07 ±2. 11) and (46.73 ± 1.14) min, respectively, and the AUC0-t was (158.52 ±6. 27), (211. 13 ±9.33) and (361.37 ± 14.97) min · mg · L-1, respectively. Conclusion A simple, rapid, accurate and precise HPLC method for the analysis of GNA in rat plasma is successfully developed and validated and successfully applied to a pharmacokinetic study of GNA in rats.关键词
新藤黄酸/大鼠/血药浓度/HPLC/测定/药代动力学Key words
gambogenic acid/ rat/ plasma concentration / HPLC / determination/ pharmacokinetics分类
医药卫生引用本文复制引用
汪电雷,张瑞,陶秀华,张弦,陈卫东,王效山,李庆林,彭代银..大鼠血浆中的新藤黄酸HPLC测定及其药代动力学研究[J].中国药理学通报,2012,28(8):1164-1167,4.基金项目
国家重大新药创制资助项目(No 2009ZX09103-399) （No 2009ZX09103-399）
国家自然科学基金资助项目(No 81001592) （No 81001592）
教育部科学技术重点项目 (No 210101) （No 210101）
安徽高校省级自然科学研究重点项目资助(No KJ2010A210,KJ2012A186) （No KJ2010A210,KJ2012A186）
