中国药理学通报2012，Vol.28Issue(8)：1173-1177,5.DOI:10.3969/j.issn.1001-1978.2012.08.030
利用斑马鱼建立成瘾性药物对运动机能及认知功能影响的评价模型
Zebrafish as a model system for assessing effects of addictive drugs on locomotor activity and cognitive performance
摘要
Abstract
Aim To establish several behavioral models to characterize the spontaneous activity and learning ability in which zebrafish was utilized as a model animal, and then to investigate the effects of addictive drugs on these models. Methods With the video record and track system, the behavior could be recorded and quantified automatically. In the experiments, the total travel distances, entries in top zone and the top/total time spent were selected as indices in locomotor model; the latency to the enriched chamber ( EC) and the total time in EC were measured in T maze test. Morphine and methamphetamine ( METH) were chosen as experimental challenges. Results In spontaneous activity test, compared to the normal group, acute morphine-treated zebrafish exhibited significant increased travel distance, vertical transitions and top time ( P < 0. 05 ) over 6 min; acute exposure of zebrafish to methamphetamine made transitions and top time decrease significantly ( P < 0. 01, P < 0. 01) . These results indicated morphine exposure induced hyperlocomotion, and a- cute methamphetamine produced robust anxiogenic effects. In T-maze test, there was a trend for normal fish to reduce the time that it took for them to reach the EC and to increase the time spent in the EC during the training period. In the group of morphine repeated exposure, the total time in EC had no significance during the training, and in the METH repeat-treated group, the space recognition ability was impaired severely. Conclusions Addictive drug treatments have effects on the motor behavior and cognition function of zebrafish. Exposed the drugs, the zebrafish exhibits characterized behavioral phenotype; the information collected from zebrafish consists with the results obtained from mice model. It suggests that zebrafish as a reliable, inexpensive model can be used as platform for assessment of neuropsychiatric disorders or injury.关键词
斑马鱼/行为模型/成瘾性药物/自发活动/认知能力/神经精神障碍Key words
zebrafish/ behavioral model/ addictive drug/ spontaneous activity/ cognitive ability/ neuropsychiatric disorders引用本文复制引用
颜慧,宫泽辉..利用斑马鱼建立成瘾性药物对运动机能及认知功能影响的评价模型[J].中国药理学通报,2012,28(8):1173-1177,5.基金项目
国家科技重大专项资助(No 2009ZX09301-002,2012ZX09301003-001-006) （No 2009ZX09301-002,2012ZX09301003-001-006）
