中国药理学与毒理学杂志2012，Vol.26Issue(2)：131-137,7.DOI:10.3867/j.issn.1000-3002.2012.02.002
网络药理学实验研究相关技术
Experimental techniques in network pharmacology
摘要
Abstract
Network pharmacology based on biological networks aims to reveal the principles of interaction between drug and organism, represents a new philosophy and patter of modern biomedical research. Network pharmacological studies at the experimental level include obtaining the basic data required by network constructing, experimental verification of the network-based prediction model for drug or drug target. The experimental techniques used in network pharmacology characterize with high-throughput, quantitative, high sensitivity and accuracy. Besides of omics (genomics, proteomics, metabolomics, glycomics, etc. ) technology, the key experimental techniques are the high-throughput/high content assay, dual high-throughput gene expression detection, molecular interaction assay. These three main technologies are reviewed in this paper.关键词
网络药理学/实验技术/高通量/高内涵技术/双高通量基因表达检测技术/分子相互作用技术Key words
network pharmacology/ experimental technique/ high throughput/high content assay/ dual high-throughput gene expression detection/ molecular interaction assay分类
医药卫生引用本文复制引用
程肖蕊,周文霞,张永祥..网络药理学实验研究相关技术[J].中国药理学与毒理学杂志,2012,26(2):131-137,7.基金项目
国家“重大新药创制”科技专项(2012ZX09301003-002-001) （2012ZX09301003-002-001）
国家科技支撑计划课题(2012BAI29B07) （2012BAI29B07）
国家重点基础研究发展计划(973计划)项目(2004CB518907) （973计划）
国家自然科学基金(30973541) （30973541）
国家自然科学基金(30772562) （30772562）
