中国药理学与毒理学杂志2012，Vol.26Issue(2)：138-144,7.DOI:10.3867/j.issn.1000-3002.2011.02.003
8-羟基喹啉酮对HepG2细胞氧化性DNA损伤的诱导作用
Inductive effect of copper 8-quinolinolate on oxidative DNA damage in HepG2 cells
林勃 1姜丽平 2耿成燕 2仲来福1
作者信息
- 1. 大连医科大学毒理学研究室,辽宁大连116044
- 2. 大连医科大学中日合作医药科学研究所,辽宁大连116044
- 折叠
摘要
Abstract
OBJECTIVE To assess the DNA damage of copper 8-quinolinolate (CuQ) and to elucidate the plausible mechanisms.METHODS HepG2 cells were treated with CuQ0-4 μmol·L-1 for different time,DNA damage was measured by Comet assay.Catalase (CAT) activity,glutathione(GSH) level and thiobarbituric acid reactive substances (TBARS) were measured.NF-κB was examined using Western blotting.8-Hydroxydeoxyguanosine (8-OHdG) was measured by immunoperoxidase staining analysis.RESULTS CuQ 0.5 -4 μmol·L-1 caused significant increase of DNA migration in HepG2 cells.CuQ significantly decreased levels of GSH and activity of CAT in HepG2 cells (P ＜0.05).Moreover,CuQ significantly increased accumulation of the p65 subunit of NF-κB into nucleus,levels of lipid peroxidation product TBARS and the formation of 8-OHdG (P ＜0.05).The intracellular GSH level was modulated by pre-treatment with buthionine-(S,R)-sulfoximine (BSO),depletion of GSH in HepG2 cells pre-treated with BSO dramatically increased susceptibility of HepG2 cells to CuQ-induced DNA damage.CONCLUSION CuQ exerts DNA damage by oxidative stress and increases accumulation of p65 subunit of NF-κB into nucleus in HepG2 cells.of GSH i关键词
8-羟基喹啉酮/氧化应激/谷胱甘肽/细胞,HepG2Key words
copper 8-quinolinolate/oxidative stress/glutathione/cells, HepG2分类
医药卫生引用本文复制引用
林勃,姜丽平,耿成燕,仲来福..8-羟基喹啉酮对HepG2细胞氧化性DNA损伤的诱导作用[J].中国药理学与毒理学杂志,2012,26(2):138-144,7.
