中国药理学与毒理学杂志2012，Vol.26Issue(2)：200-204,5.DOI:10.3867/j.issn.1000-3002.2012.02.014
当归多糖对幼年大鼠染铅所致贫血的治疗作用
Effect of Angelica polysaccharides on anemia of young rats exposed to lead
摘要
Abstract
OBJECTIVE To investigate effect of Angelica polysaccharides (APS) on expelling lead and on anemia of young rats exposed to lead. METNODS Four-week-old SD rats were ip given lead acetate 30 mg·kg-1 every other day, for 14 d. APS 15 , 30, 60 and 120 mg·kg-1 were ig given on the 2nd day after model established, once daily. On the day before drug, the 7th and 14th days after drug, blood samples were collected and blood lead ( BPb) , hemoglobin ( Hb) and red blood cells (RBC) were detected. On the 14th day after drug all the rats were sacrificed and RBC hematocrit (Hct), 8-aminolevulinic acid dehydratase (ALAD) activity and zinc original porphyrin (ZPP) concentration were detected. BPb level was detected by graphite furnace atomic absorption spectrometry. Hb content was detected by GaoTieFa cyanide. RBC count was tested by microscope count method. Hct, ALAD activity and ZPP concentration were detected by blood analyzer, parad-imethylamino-benzaldehyde colorimetric method and zinc porphyrin blood fluorescence detector, respectively. RESULTS Compared with normal group, BPb content in model group on the day before drug, 7th and 14th day significantly increased, and Hb and RBC decreased(P <0.01). Compared with model group, BPb contents in APS 15 -120 mg· kg-1 groups on the 7th day and 14th day significantly decreased, but still higher than those in corresponding normal group(P <0. 01). Hb and RBC in APS 15 - 120 mg·kg-1 on the 7th day and the 14th day significantly increased(P < 0.01). Moreover, compared with the 7th day, Hb increased by 33. 4% , 34. 1% , 21. 0% and 34.6% , RBC increased 28. 5% , 17. 9% , 18. 3% and 19. 2% on the 14th day in APS 15 -120 mg·kg-1 groups, respectively ( P < 0. 01). Compared with model group, Hct in APS 15 — 120 mg·kg-1 groups on the 14th day significantly increased(P <0. 01) , and was similar to that in normal group. ALAD significantly increased ( P < 0. 01). Meso-2, 3-Dimercaptosuccinic acid 120 mg·kg· had the same effects as APS on the anemia model. CONCLUSION APS has the obvious effect on expelling lead and can improve anemia of young rats exposed to lead.关键词
当归多糖/血铅/贫血Key words
Angelica polysaccharide/ blood lead/ intervention/ anemia分类
医药卫生引用本文复制引用
田丹,王敏,李程,王穆,谭伟,刘佩意,让蔚清..当归多糖对幼年大鼠染铅所致贫血的治疗作用[J].中国药理学与毒理学杂志,2012,26(2):200-204,5.基金项目
湖南省卫生厅科研项目(2010-203) （2010-203）
湖南省衡阳市社会基金资助项目(2011-KF4) （2011-KF4）
全国教育科学“十一五”规划2010年度教育部重点课题(GLA102077) （GLA102077）
