中国药理学与毒理学杂志2012，Vol.26Issue(2)：231-236,6.DOI:10.3867/j.issn.1000-3002.2012.02.019
朱砂安神丸、朱砂、硫化汞和氯化汞对大鼠肝脏细胞色素P450酶的影响
Effect of cinnabar, Zhusha Anshenwan, HgS and HgCl2 on expression of cytochrome P450 in livers of rats
摘要
Abstract
OBJECTIVE To explore effect of cinnabar and cinnabar-containing Zhusha Anshenwan on cytochrome P450( CYP) mRNA in liver of rats. METHODS Adult Sprague-Dawley rats were gavaged with Zhusha Anshenwan 1.4 g · kg-1, cinnabar 0.15 g·kg-1, HgS 0. 15 g · kg-1, HgCl20.02 g· kg-1, once daily, for 21 d, and liver toxicity was determined by measuring body mass gain. Liver index, alanine transaminase (ALT) ativity, mercury accumulation in liver and mRNA levels of metalloehionein-2(MT-2), CYP1A, CYP3A2, CYP4A10, CYP2B1 and constitute andro-stane receptor (CAR) genes were detected with RT-PCR. RESULTS Compared with normal control group, body mass gain retarded in HgCl2 group with a reduced food intake and activities. A significant accumulation of Hg in livers in HgCl2 group was evident, however these changes were not evident in cinnabar, Zhusha Anshenwan and HgS groups. Compared with normal control group, mRNA expressions of MT-2, CYP1A1, CYP1A2 were evidently higher and CAR were apparently lower in HgCl2 group. Compared with nromal control group, accumulation of Hg, liver index, ALT, MT-2, CYP1A1, CYP1A2 and CYP4A10 CAR, CYP2B1 mRNA in cinnabar, Zhusha Anshenwan and HgS groups were unaltered, except that CYP3A2 mRNA expression obviously increased in cinnabar group. CONCLUSION Zhusha Anshenwan, cinnabar, and HgS accumulate much less mercury in the liver than HgCl2 at clinical dose for 3 weeks. MT-2, CAR and cytochrome P450 genes are altered in HgCl2 group, CYP3A2 mRNA increase in cinnabar group, but all of above are not changed in Zhusha Anshenwan and HgS groups.关键词
朱砂/朱砂安神丸/肝/金属硫蛋白/细胞色素P450酶系统/受体,胞质和核Key words
Cinnabar/ Zhusha Anshenwan/ liver/ metallothionein/ cytochrome P450 enzyme system/ receptors, cytoplasmic and nuclear分类
医药卫生引用本文复制引用
杨虹,彭芳,刘杰,吴芹,时京珍..朱砂安神丸、朱砂、硫化汞和氯化汞对大鼠肝脏细胞色素P450酶的影响[J].中国药理学与毒理学杂志,2012,26(2):231-236,6.基金项目
贵州省国际科技合作项目(G2011-7020) （G2011-7020）
贵州省中药现代化项目(2010-5) （2010-5）
