中国药理学与毒理学杂志2012，Vol.26Issue(3)：340-343,4.DOI:10.3867/j.issn.1000-3002.2012.03.015
高效液相色谱法测定吡美拉唑血药浓度及其药代动力学
Determination of pymeprazole in human plasma by RP-HPLC and its pharmacokinetics
摘要
Abstract
OBJECTIVE To establish a high performance liquid chromatography (HPLC) method for pymeprazole in humans, and to explore its pharmacokinetics. METHODS HPLC column was Diamond C18(5 μm, 250 mm ×4. 6 mm) column, the mobile phase was 0. 05 mol·L-1 phosphatic buffer (pH =6.50)-acetonitrile (64:36, V/V) , flow rate was 1.0 ml-min"1, and UV detection wavelength was set at 305 nm. Subjects were given pymeprazole 10 mg (po) before blood samples were collected at 0, 0. 5 , 1, 1.5,2,4,8, 12, 24, 36, 48, 60 and 72 h after administration. Concentrations of pymeprazole in plasma were determined by HPLC, and parameters were calculated with 3P87 software. RESULTS The calibration curve of pymeprazole in plasma samples was linear over the range of 25 - 4000 μg · L-1 ( r = 0. 99998). The lower limit of quantification for pymeprazole in plasma was 25μg·L-1. The recovery of the method was from 95. 2% to 107. 7%. The intra-day RSD and inter-day RSD were less than 6. 9% and 10. 2% , respectively. The main pharmacokinetic parameters of pymeprazole were t1/2 (22. 58 ± 1. 59) h, AUC0_72 (29 089 ± 8886)μg·h·L-1 CI/F (338.9 ± 114. 0)L·h-1, tmax(2.67 ±1.54)h, and Cmax was (1585 ± 469)μg·L-1. CONCLUSIONS HPLC method is simple, quick, sensitive and accurate. Pymeprazole is rapidly absorbed, and its t1/2 is longer than that of other proton inhibitors in subjects.关键词
吡美拉唑/高效液相色谱法/药代动力学Key words
pymeprazole/ HPLC/ pharmacokinetics分类
医药卫生引用本文复制引用
孙黎,苏克剑,姚晓东,茅益民..高效液相色谱法测定吡美拉唑血药浓度及其药代动力学[J].中国药理学与毒理学杂志,2012,26(3):340-343,4.基金项目
国家科技部“重大新药创制”科技重大专项(2008ZX09312-007) （2008ZX09312-007）
