中国药业2012，Vol.21Issue(14)：58-59,2.
高效液相色谱法测定门冬氨酸洛美沙星氯化钠注射液的含量
Determination of Lomefloxacin Aspartate and Sodium Chloride Injection by HPLC
赵卉 1杨菲 1樊红梅 1吴沂1
作者信息
- 1. 陕西金裕制药股份有限公司,陕西,西安,710075
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the enantiomers content in valsartan capsules. Methods The α — acidic glycoprotein column ( AGP, 100 mm × 4. 0 mm, 5 μm) was adopted with phosphate buffer ( taking sodium dihydrogen phosphate 2.51 g and potassium dihydrogen phosphate 1.91 g, adding water for dissolution and diluting to 1 000 mL, adjusting pH 7.0 with 1 mol/L sodium hydroxide solution) —isopropanol (98:2) as the mobile phase. The detecting wavelength was 227 nm, the flow rate was 0. 8 mL/min and the column temperature was 30 ℃. Results The enantiomers in valsartan capsules showed a good linear relation in the range of 0.009 44-0.188 8μg. The detecting limit was 1. 510 4 ng and the quantitative limit was 3. 398 4 ng. Conclusion This method is simple, accurate, sensitive, strongly specific and applicable to examine enantiomers in valsartan capsules.关键词
高效液相色谱法/门冬氨酸洛美沙星/含量测定Key words
HPLC/valsartan capsules/enantiomers分类
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赵卉,杨菲,樊红梅,吴沂..高效液相色谱法测定门冬氨酸洛美沙星氯化钠注射液的含量[J].中国药业,2012,21(14):58-59,2.
