中国中医药信息杂志2012，Vol.19Issue(7)：52-53,2.DOI:10.3969/j.issn.1005-5304.2012.07.021
RP-HPLC测定止痛膏中大黄酸、大黄素和大黄酚的含量
Content Determination of Rhein, Emodin and Chrysophanol in Zhitonggao by RP-HPLC
摘要
Abstract
Objective To establish the method to determine the contents of rhein, emodin and chrysophanol in Zhitonggao. Methods RP-HPLC method was adopted with Hypersil ODS C18 as column. The mobile phase consisted of methanol-water (85∶15) with a flow rate of 1.0 mL/min. The detection wavelength was 254 nm. Results The linear correlation of rhein was good in the range of 0.208－1.040 μg (r ＝0.999 0, n＝5) and the recovery was 98.37% (RSD＝0.57%, n＝5). The linear correlation of emodin was good in the range of 0.192－0.96 μg (r ＝0.999 7, n＝5) and the recovery was 98.45% (RSD＝0.99%, n＝5). The linear correlation of chrysophanol was good in the range of 0.596－2.980 μg (r ＝0.999 5, n＝5) and the recovery was 98.18% (RSD＝1.50%, n＝5). Conclusion The method is simple, sensitive and accurate, with good reproducibility. It can be applied to control the quality of the preparation.关键词
止痛膏/大黄酸/大黄素/大黄酚/反相高效液相色谱法Key words
Zhitonggao；rhein；emodin；chrysophanol；RP-HPLC分类
医药卫生引用本文复制引用
闵云山,焦正花,张小华,杨晔..RP-HPLC测定止痛膏中大黄酸、大黄素和大黄酚的含量[J].中国中医药信息杂志,2012,19(7):52-53,2.基金项目
甘肃省自然科学基金(1010RJZ206) （1010RJZ206）
