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响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺

王银存 王卫卫 李利军 马英辉 王小娟 卢美欢

中国造纸学报2012,Vol.27Issue(2):45-49,5.
中国造纸学报2012,Vol.27Issue(2):45-49,5.

响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺

Optimization of Gluconacetobacter Fermentation for Bacterial Cellulose Production by Response Surface Method

王银存 1王卫卫 2李利军 2马英辉 1王小娟 1卢美欢1

作者信息

  • 1. 陕西省微生物研究所,陕西西安710043
  • 2. 西北大学生命科学学院,陕西西安,710069
  • 折叠

摘要

Abstract

In order to increase bacterial cellulose production yielded by Gluconacetobacter, the factors including sweet potato hydrolyzate, initial pH, liquid volume in flask and so on by using Plackett-Burman Design and Box-Benhnken Design combination were studied, and the fermentation of bacterial cellulose production was optimized. The results showed that the best conditions for bacterial cellulose production were as follows: sweet potato hydrolyzate of 50 g/L, initial pH 6.0, liquid volume in flask of 50 ml/ (250 mL). Under these conditions, the dry weight of the bacterial cellulose reached to 4.80 g/L and increased by 118% compared with 2.20 g/L before optimization.

关键词

响应面法/细菌纤维素/发酵工艺/葡糖醋杆菌

Key words

response surface method/ bacterial cellulose/ fermentation/Gluconacetobacter

分类

轻工纺织

引用本文复制引用

王银存,王卫卫,李利军,马英辉,王小娟,卢美欢..响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺[J].中国造纸学报,2012,27(2):45-49,5.

基金项目

陕西省科学院青年人才基金(2010k-29). (2010k-29)

中国造纸学报

OA北大核心CSCDCSTPCD

1000-6842

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