浙江农林大学学报2012，Vol.29Issue(2)：155-160,6.
小鼠mdm2的表达及活性鉴定
Expression and activity analysis of mouse mdm2
摘要
Abstract
The mdm2 (murine double minute 2) is one of the factors inhibiting p53 activity, and their interaction is important to hold cell survival. In this paper, mouse mdm2 gene was cloned from mouse 3T3 cell strain, and the recombinant plasmid p3xFLAG-CMV-7.1-mdm2 was constructed and confirmed by restriction enzyme digestion and DNA sequencing. Western blotting analysis and indirect immune-fluorescent analysis showed that FLAG-mdm2 expressed in p3xFLAG-CMV-7.1-mdm,2-transfe,cted H1299 cells, which was 60 kDa in size and dominantly localized in nucleus. The activity of FLAG-mdm2 protein was confirmed by p53 ubiqui-tination assay and luciferase assay. The result showed that mdm2 cell model inhibiting p53 activity is established'. Our results would be useful for studying the mdm2 biological fuction in regulating p53 activity and interaction between mdm2 and p53. [Ch, 5 fig. 15 ref. ]关键词
动物学/mdm2表达/mdm2活性/细胞模型Key words
zoology/ mdm2 expression/ mdm2 acitivity/ cell model分类
生物科学引用本文复制引用
王晓杜,赵阿勇,邓绪芳,马志永..小鼠mdm2的表达及活性鉴定[J].浙江农林大学学报,2012,29(2):155-160,6.基金项目
浙江省自然科学基金资助项目(Y3110124) （Y3110124）
浙江农林大学人才启动基金资助项目(2010FR080) （2010FR080）
