浙江农林大学学报2012，Vol.29Issue(2)：244-250,7.
雷竹林存留有机覆盖物高效降解菌株分离及产酶条件优化
Selection of strains used to degrade organic mulching materials from Phyllostachys violascens forest and optimization of its enzyme production
摘要
Abstract
Severe degradation of a Phyllostachys violascens forest has occurred from organic mulch residues for years. To artificially promote decomposition of organic mulching material and promote regeneration of bamboo forest, seven cellulase-producing strains, including 4 fungi and 3 actinomycetes, from organic matter and soil of a Ph. violascens forest were chosen using a Congo red cellulose identification culture medium. A filter paper enzyme activity (FPA) as well as colony morphology and biological characteristics were used for the analysis. Re suits showed that among all selected strains, the FPA of Penicillium spp. (Strain 2.1) was highest. Optimal fer mentation conditions were as follows: an initial pH 5.0, a cultivation temperature of 30 t, and a fermentation time of 5 d. The optimal cultivation medium for Strain 2.1 was chosen as; straw powder (25.00 g·L-1) for the C source, beef extract (2.50 g·L-1) for the N source, and FeCl3 (0.01 g·L-1) and KH2PO4 (1.50 g·L-1) for important inorganic salts. [Ch, 4 fig. 3 tab. 20 ref.]关键词
微生物学/雷竹/有机覆盖物/降解菌株/纤维素降解酶/产酶条件Key words
microbiology/ Phyllostachys violascens/ organic mulching material/ decomposing strain/ cellu-lase/ enzyme production分类
农业科技引用本文复制引用
可晓,陈双林,张小平,郭子武..雷竹林存留有机覆盖物高效降解菌株分离及产酶条件优化[J].浙江农林大学学报,2012,29(2):244-250,7.基金项目
浙江省林业科技推广项目(2011B01) （2011B01）
杭州市科研院所专项(20090332N01) （20090332N01）
