肿瘤防治研究2012，Vol.39Issue(4)：385-388,4.DOI:10.3971/j.issn.1000-8578.2012.04.005
内质网应激对乳腺癌MCF-7细胞CCL5表达的影响
Endoplasmic Reticulum Stress-induced Overexpression of CCL5 in Human Breast Cancer Cell MCF-7
范威 1潘翠萍 2张懿敏 1廖仕翀 1魏文 3马彪 2孙圣荣3
作者信息
- 1. 430060,武汉,武汉大学第一临床学院乳甲外科
- 2. 湖北省肿瘤医院乳腺科
- 3. 湖北省人民医院乳甲外科
- 折叠
摘要
Abstract
Objective To discuss the relationship between ER stress and the expression of CCL5 in human breast cancer cell MCF-7 and identify the correlation between CCL5 and the proliferation and metastasis capacity of human breast cancer MCF-7 cells. Methods The ER stress and the expression of CCL5 in the tissue of human breast cancer and adjacent tissue were datected by Western blot. ER stress inducer Tu-niamycin and ER stress inhibitor 4-PBA were used to MCF-7 cells respectively. Samples were collected the total cell protein after 24 hours treatment. Tthe ER stress and the expression of CCL5 in MCF-7 cells was analyzed by Westernblot. The proliferation and metastasis capacity of human breast cancer MCF-7 cells were measured by MTT colorimetry and transwell experiment respectively. ELISA was employed to detect the content of CCL5 in the culture medium. Results The ER stress and the expression of CCL5 in the tissue of human breast cancer were in higher level than that in adjacent tissue. The ER stress of the cells treated with ER stress inducer was in higher level and these cells expressed more CCL5. On the contrary, the ER stress of the cells treated with ER stress inhibitor was in lower level and these cells expressed less CCL5. In addition,the cells treated with ER stress inducer were more proliferative than the cells treated with ER stress inhibitor . CCL5 secreted to the culture medium could enhance the capacity of proliferation and metastasis of human breast cancer MCF-7 cells. Conclution ER stress can induce the endogenous expression of CCL5 in MCF-7 cells. Endogenous CCL5 can promote the proliferation of human breast cancer MCF-7 cells and extraneous CCL5 can promote the metastasis of MCF-7 cells.关键词
乳腺癌/内质网应激/CCL5Key words
Breast cancer/Endoplasmic reticulum stress/CCL5分类
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范威,潘翠萍,张懿敏,廖仕翀,魏文,马彪,孙圣荣..内质网应激对乳腺癌MCF-7细胞CCL5表达的影响[J].肿瘤防治研究,2012,39(4):385-388,4.
