肿瘤防治研究2012，Vol.39Issue(4)：400-402,3.DOI:10.3971/j.issn.1000-8578.2012.04.008
Cdx2过表达对裸鼠人胃癌移植瘤生长和转移的影响
Effects of Cdx2 Overexpression on Growth and Metastasis of Transplanted Tumor of Human Gastric Cancer in Nude Mice
摘要
Abstract
Objective To study the effects of Cdx2 overexpression on growth and metastasis of transplanted tumor of human gastric cancer in nude mice. Methods The recombined eukaryotic expression vector pCMV-Cdx2-HA or empty vector pCMV-HA was transfected into gastric cancer MGC-803 ceils individually by Lipofectamine, which was named as MGC-803/Cdx2 cells or MGC-8K3/EV cells. BALB/C nude mice were inoculated subcutaneously with MGC-803/Cdx2 cells or MGC-803/EV cells and then the subcutaneous tumor was transplanted into stomach. The nude mice transplanted with MGC803/Cdx2 tumor were named as the experimental group while the nude mice transplanted with MGC-803/EV tumor were named as the control group. After mice were executed,the volume o! transplanted tumor was measured and tumor metastasis was observed. The expression of Cdx2 in tumor tissue was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Results The volume of transplanted tumor in the experimental group was (13. 42 ± 2. 34) mm3, which was lower than that in the control group (P<(). 05). Meanwhile, the rate of tumor formation in the experimental group (73. 3%) was lower than that in the control group (86. 7%). The situation of tumor metastasis was not difference between the control group and the experimental group (P>0. 05). Compared with the control group.both Cdx2 mRNA and protein were upregulated in the experimental group (P"). 05). Conclusion Cdx2 overexpression inhibites growth of transplanted tumor of human gastric cancer in nude mice, but it has no effect on tumor metastasis.关键词
Cdx2/胃癌/移植瘤Key words
Cdx2/Gastric cancer/Transplanted tumor分类
医药卫生引用本文复制引用
廉超,王晓通,谢玉波,肖强..Cdx2过表达对裸鼠人胃癌移植瘤生长和转移的影响[J].肿瘤防治研究,2012,39(4):400-402,3.基金项目
国家自然科学基金资助项目(81060201) （81060201）
广西自然科学基金资助项目(2011GXNSFA018273) （2011GXNSFA018273）
高等学校博士学科点专项科研基金资助项目(20114503110002) （20114503110002）
