肿瘤防治研究2012，Vol.39Issue(5)：502-505,4.DOI:10.3971/j.issn.1000-8578.2012.05.003
NDV7793体外激活的小鼠单核巨噬细胞(MΦ)对小鼠肝癌细胞的杀伤作用及其机制
Killing Effect on Liver Cancers by Mouse Macrophages Stimulated by Newcastle Disease Virus 7793 Strain in vitro and Its Mechanism
摘要
Abstract
Objective To study the killing effect on liver cancers by mouse macrophages stimulated by Newcastle disease virus 7793 strain in vitro and the association of TRAIL. Methods The BALB/C mouse macrophages were harvested by using peritoneal lavage. And then the mouse macrophages were stimulate in -vitro by NDV7793. The concentration of TNF-α and TRAIL was determined by ELISA after NDV stimulation. Then the macrophages of mice were coincubated and activated with Novikoff cells. The cytotoxic effect of macrophages on Novikoff cells was performed by Lactate Dehydrogenase(LDH) assay after NDV stimulation. Three experiment control groups were simultaneously set up as following:TFN-β positive control group.ultraviolet ray inactivated NDV(UV-NDV) control group as well as blank control group. Results Compared with three control groups in vitro,the macrophages stimulated with NDV 7793 had been activated,and the level of TNF-a and TRAIL in culture supernatant increased. The killing ability of macrophage to Novikoff cells after NDV stimulation had increased. Conclusion The NDV 7793 can activate the mouse macrophages in vitro. The killing effect on liver cancer cells of the mouse macrophages is enhanced by NDV stimulation. And it is possible that TRAIL and TNF-a involve in this may enhance the killing effect.关键词
NDV 7793/小鼠/单核-巨噬细胞/肝癌细胞Key words
NDV 7793/ Mouse/Mononuclear macrophage/Liver cancer cells分类
医药卫生引用本文复制引用
刘金颖,赖振屏,宫金伶,樊晓晖,宋德志,王立芳,潘文宝胜,殷君,梁莹,肖庆..NDV7793体外激活的小鼠单核巨噬细胞(MΦ)对小鼠肝癌细胞的杀伤作用及其机制[J].肿瘤防治研究,2012,39(5):502-505,4.基金项目
国家自然科学基金资助项目(30860328) （30860328）
广西自然科学基金资助项目(2011GXNSFA01822) （2011GXNSFA01822）
广西教育厅科学基金资助项目(200810MS059) （200810MS059）
广西研究生教育创新计划资助项目(2010105981002M226) （2010105981002M226）
