中山大学学报（医学科学版）2012，Vol.33Issue(3)：351-356,6.
β溶血性链球菌htra基因的测序鉴定及表达蛋白的疫反应性分析
Identification of htra Gene and Immunoreactivity Analysis of htrA Protein in Beta hemolytic Streptococci
摘要
Abstract
[Objective] To investigate whether hlra homologous genes were present in the 44 BHS isolates from children with acute throat infection or tonsillitis in Guangzhou recently and analyze the immunoreactivity of htra protein. [Methods] Htra genes were amplified by polymerase chain reaction (PCR) and identified by sequencing. Then the htra gene from GAS was cloned into pGEX4T-l vector and HtrA protein was expressed in E.coli BL21. The recombinant HtrA protein was identified by Western blot with anti-GST rabbit mAb and its immunoreactivity was analyzed by Western blot with the sera from mice infected with BHS. [Results] All of the 44 BHS isolates harboured the htra genes which were 99% identical with the gene of GAS. Western blot confirmed that both anti-GST rabbit mAb and the sera from mice infected with GAS could react specifically with the recombinant HtrA protein, while the sera from other group could not. [Conclusions] All of the BHS isolates contain htra gene which is the same as the known GAS gene and is different from the known GBS and GCS gene. GAS infection is able to induce antibody against HtrA protein. This indicates that HtrA protein acts as a dominant immunogen in GAS, while the immunoreactivity of HtrA proteins from other groups of streptococci is still unknown.关键词
β溶血性链球菌/htra基因/克隆表达/免疫反应性Key words
beta-hemolytic streptococci/htra gene/protein expression/immunoreactivity分类
生物科学引用本文复制引用
倪琼琼,丁月霞,刘金来,余步云..β溶血性链球菌htra基因的测序鉴定及表达蛋白的疫反应性分析[J].中山大学学报（医学科学版）,2012,33(3):351-356,6.基金项目
广东省科技计划项目(2008B080701023) （2008B080701023）
