作物研究2012，Vol.26Issue(3)：205-208,223,5.DOI:10.3969/j.issn.1001-5280.2012.03.01
AtCS25基因过表达载体的构建和拟南芥的遗传转化
Construction of Overexpression Vector of AtCS25 Gene and Its Transformation in Arabidopsis thaliana
摘要
Abstract
AtCS25 is a function unknown gene in Arabidopsis thaliana. According to the analysis result of bioinformatics, this gene may associate with pollen germination and tube growth. In order to uncover the function of this gene, the full length CDS of AtCS25 was cloned into overexpression binary vector Pbi121 and was transformed into wild - type Arabidopsis thaliana Col mediated by Agrobacterium tumefaciens GV3101. Five transgenic plants were obtained, which played a good foundation for further studying on function of AtCS25 .关键词
AtCS25:生物信息学分析:过表达载体构建/遗传转化Key words
AtCS25 / Bioinformation analysis/ Construction of over expression vector/ Genetic transformation分类
生物科学引用本文复制引用
刘博宇,阮颖..AtCS25基因过表达载体的构建和拟南芥的遗传转化[J].作物研究,2012,26(3):205-208,223,5.基金项目
国家自然科学基金(31071455). （31071455）
