作物学报2012，Vol.38Issue(6)：962-970,9.DOI:10.3724/SP.J.1006.2012.00962
利用关联分析发掘小麦自然群体旗叶叶绿素含量的优异等位变异
Exploring Elite Alleles for Chlorophyll Content of Flag Leaf in Natural Population of Wheat by Association Analysis
摘要
Abstract
Elite alleles associated with chlorophyll content of flag leaf in a natural population of winter wheat (Triticum aestivum L.) consisting of 262 accessions were measured at flowering and grain-filling stages under both rainfed and well-watered conditions in two experimental locations in Beijing, China. A total of 169 SSR markers distributed on the 21 chromosomes of wheat were employed to detect the genetic diversity and genetic structure of the population. Association analysis between SSR loci and chlorophyll content trait was performed using TASSEL MLM (mixed linear model) program. The phenotypic allele effect was estimated through comparing the average phenotypic value over accessions with that of "null allele". A total of 2048 alleles were identified on the 169 SSR loci, and each locus had 2-37 alleles with an average of 12. The polymorphism information contents (PICs) of the SSR loci ranged from 0.008 to 0.936 with an average of 0.628. Forty markers were found to be significantly associated with chlorophyll content (P<0.001) on 22 loci, of which 11 markers showed repeated associations, particularly, loci Xwmc419-1B and Xgwm501-2B had associations for three times. The alleles on loci Xcfa2123-7A, Xgwm232-1D, and Xgwm429-2B exhibited more than 4.0 of phenotypic effect value.关键词
小麦/叶绿素含量/SSR/关联分析/等位变异Key words
Wheat (Triticum aestivum L.)/ Chlorophyll content/ SSR/ Association analysis/ Allelic variation引用本文复制引用
李玮瑜,张斌,张嘉楠,昌小平,李润植,景蕊莲..利用关联分析发掘小麦自然群体旗叶叶绿素含量的优异等位变异[J].作物学报,2012,38(6):962-970,9.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2011AA100501)和国际农业研究磋商组织(CGIAR)挑战计划项目(G7010.02.01)资助. （863计划）
