作物学报2012，Vol.38Issue(6)：971-979,9.DOI:10.3724/SP.J.1006.2012.00971
野生大豆GsGST19基因的克隆及其转基因苜蓿的耐盐碱性分析
Isolation of GsGST19 from Glycine soja and Analysis of Saline-Alkaline Tolerance for Transgenic Medicago sativa
摘要
Abstract
Alfalfa (Medicago sativa L.) is one of the most important leguminous forage crops worldwide. Saline-alkaline stress significantly limits the productivity of alfalfa due to its adverse effects on growth, formation of nodules, and symbiotic nitrogen-fixation capacity, and resulting in the formation of reactive oxygen species (ROS). To protect plants from the toxicity of reactive oxygen, aerobic organisms are equipped with an array of defense mechanisms, including one based on glutathione 5-transferases (GSTs). Glutathione S-transferases (GSTs) are ubiquitous enzymes that play a key role in stress tolerance and cellular detoxification. The GST gene CsGST19 isolated from wild type soybean (Glycine soja) under saline-alkaline stress was transformed into alfalfa (Medicago sativa L.). Transgenic alfalfa plants showed 0.52-0.49 times higher levels of GST activity than wild type plants. Transgenic alfalfa grew well in the conditions of 100 mmol L-1 NaHCO3, while wild type plants exhibited discoloration and stunted growth, or even death. There were significantly changes in malondialdehyde content and relative membrane permeability caused by saline-alkaline stress in non-transgenic lines compared to transgenic lines (P<0.05). Moreover, compared with non-transgenic, transgenic alfalfa had higher levels of chlorophyll content and root activity under saline-alkali stress conditions. The results indicated that the gene GsGSTW could enhance resistance to saline-alkaline in alfalfa.关键词
谷胱甘肽S-转移酶(GST)/转基因苜蓿/野大豆/耐盐碱性Key words
Glutathione S-transferase/ Transgenic alfalfa/ Glycine soja/ Saline-alkaline stress引用本文复制引用
王臻昱,才华,柏锡,纪巍,李勇,魏正巍,朱延明..野生大豆GsGST19基因的克隆及其转基因苜蓿的耐盐碱性分析[J].作物学报,2012,38(6):971-979,9.基金项目
本研究由国家自然科学基金项目(31171578),黑龙江省高校科技创新团队建设计划(2011TD005),国家高技术研究发展计划(863计划)项目(2008AA10Z153)和黑龙江省科技厅重大攻关项目(GA06B103)资助. （31171578）
