作物学报2012，Vol.38Issue(6)：980-987,8.DOI:10.3724/SP.J.1006.2012.00980
水稻稻瘟病菌诱导表达启动子OsQ16p的克隆与功能分析
Cloning and Functional Analysis of Magnaporthe oryzae-Induced Promoter OsQ16p in Rice
摘要
Abstract
The qRT-PCR analysis showed that, in Nipponbare (Oryza sativa L. Ssp japonica), the expression of OsQ16 gene was induced by Magnaporthe grisea. The 1 299 bp-fragment of 5'-end of OsQl6 gene, named as OsQ16p, was amplified by PCR from Nipponbare. The plasmids pBIQ16p was constructed by replacing the CaMV35S promoter of pBI121 with the OsQ16p, and transformed into Nipponbare through Agrobacterium-medistlzd transformation. The analysis of GUS activity and qRT-PCR showed that gus gene could express in transgenic plants and calli. The expression of gus gene in transgenic plants was obviously enhanced by M. Grisea. After treatment with the resistance-related signaling molecules SA and MeJA, the GUS activities in transgenic plants were increased by 3.1- and 3.5-fold, respectively. It suggested that M. Grisea, SA, and MeJA were inductive factors of OsQ16p promoter.关键词
转基因水稻/诱导型启动子/OsQ16p/GUS/稻瘟病菌Key words
Transgenic rice/ Inducible promoter/ OsQ16p/ GUS/ Magnaporthe grisea引用本文复制引用
王光,吴智丹,张磊,刘凤权,邵敏..水稻稻瘟病菌诱导表达启动子OsQ16p的克隆与功能分析[J].作物学报,2012,38(6):980-987,8.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2007AA10Z188,2008AA10Z108)和国家转基因生物新品种培育项目(2009ZX08001005B)资助. （863计划）
