中国中西医结合杂志2012，Vol.32Issue(4)：486-489,4.
枳术通便汤对慢传输型便秘大鼠结肠墨汁推进率、GDNF及NOS mRNA表达的影响
Effects of Zhizhu Tongbian Decoction on the Colon Ink Propelling Rate, GDNF, and NOS mRNA Expression In Rats with Slow Transit Constipation
摘要
Abstract
Objective To observe the effects of Zhizhu Tongbian Decoction (ZTD) on the enteric nervous system,mRNA expressions of glial cell line derived neurotrophic factor (GDNF) and nitric oxide synthase (NOS) in the slow transit constipation (STC) rats. Methods Thirty STC rat model was established by gastric irrigation of rhubarb. After the model building, they were randomly divided into three groups, i. e., the model group, the high dose ZTD group, and the low dose ZTD group, 10 in each. Another 10 rats were selected as the blank control group. Rats in the high dose ZTD group and the low dose ZTD group were administered with ZTD (at the daily dose of crude drug 4.8 g/kg and 2.4 g/kg respectively) by gastrogavage. Normal saline was given to rats in the blank control group and the model group. The ink propelling rate was determined using ink propelling test. Meantime, mRNA expressions of GDNF and NOS in the rat colon were measured using reverse transcrip-tional polymerase chain reaction (RT-PCR). Results Compared with the blank control group, the ink propelling rate and GDNF mRNA expression decreased, and NOS mRNA increased in the model group, showing statistical difference (P<0.01, P<0.05). Compared with the model group, the ink propelling rate increased in the high and low dose ZTD groups (P<0.01, P<0.05). The mRNA expressions of GDNF increased and the mRNA expressions of NOS decreased in the high dose ZTD group with statistical difference (P < 0.01, P < 0.05). But there was no difference in any index between the high and low dose ZTD groups. Conclusion High dose ZTD could obviously improve the intestinal transmission function possibly through up-regulating the mRNA expressions of GDNF and down-regulating the mRNA expressions of NOS in STC rats.关键词
慢传输型便秘/枳术通便汤/墨汁推进率/结肠神经营养因子/一氧化氮合酶Key words
slow transit constipation/ Zhizhu Tongbian Decoction/ ink propelling rate/ glial cell line derived neurotrophic factor/ nitric oxide synthase引用本文复制引用
范一宏,徐国萍,冯雯,吕宾..枳术通便汤对慢传输型便秘大鼠结肠墨汁推进率、GDNF及NOS mRNA表达的影响[J].中国中西医结合杂志,2012,32(4):486-489,4.基金项目
浙江省中医药管理局资助项目(No.2010ZB039) （No.2010ZB039）
