中国中西医结合杂志2012，Vol.32Issue(4)：499-503,5.
灵猫方抑制饥饿诱导HepG 2.2.15细胞自噬研究
Lingmao Recipe Inhibits Starvation Induced Autophagy in HepG2 2.15 Cell
摘要
Abstract
Objective To study the effects of LJngmao Recipe (LR) on starvation-induced autophagy in HepG 2.2.15 cell. Methods Fifteen SD rats were selected to prepare LR drug serum. The Earle's balanced salt solution (EBSS) group, the EBSS + vehicle serum group, the EBSS +5 times LR serum group, the EBSS +10 times LR serum group, and the DMEM group were set up, 3 samples in each group. The HepG2.2.15 cells were cultured for 1,2, 4, and 6 h respectively. The pEGFP-N1 -LC3B eukaryotic expression vector was constructed and transfected with HepG2.2.15 cell. The GFP-LC3B morphological changes were observed under fluorescent microscope. The ratio of the dotty GFP-LC3B number and the GFP-LC3B transfected HepG2.2.15 number was calculated. The intracytoplasma autophagosome changes were observed using electronic transmission electron. The microtubule-associated protein 1 light chain 3 beta Ⅱ/ Ⅰ was detected in HepG2.2.15 of each group u-sing Western blot. Results Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). The electronic transmission electron showed that the formation of autophagosome was inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group. Results of Western blot showed that microtubule-associated protein 1 light chain 3 beta Ⅱ / Ⅰ obviously decreased more in the EBSS +10 times LR serum group than in the EBSS + vehicle serum group (P <0.01). Conclusion LR could inhibit starvation-induced autophagy in HepG 2.2.15.关键词
灵猫方/HepG2.2. 15细胞/自噬Key words
LJngmao Recipe/ HepG2.2.15 cell/ autophagy引用本文复制引用
朱晓骏,孙学华,刘顺庆,李曼,商斌仪,杨婉凤,高月求..灵猫方抑制饥饿诱导HepG 2.2.15细胞自噬研究[J].中国中西医结合杂志,2012,32(4):499-503,5.基金项目
国家“十一五”课题重大科技专项资助项目(No.20082X10005-006) （No.20082X10005-006）
国家自然科学基金资助项目(No.81072792) （No.81072792）
上海高校创新团队建设项目(第1期) （第1期）
上海市教育委员会重点学科资助项目(No.J50307) （No.J50307）
上海市科委优秀学科带头人(No.10XD1404100) （No.10XD1404100）
上海市教育委员会E研究院建设计划项目(No.03008) （No.03008）
上海市自然基金资助项目(No.10ZR1430900) （No.10ZR1430900）
上海市卫生局课题资助项目(No.2010QL007A) （No.2010QL007A）
上海中医药大学优秀团队培养计划(第1期) （第1期）
