中国中西医结合杂志2012，Vol.32Issue(4)：510-514,5.
丹皮酚、芍药苷及二者配伍对体外培养心肌细胞缺氧/复氧损伤的影响
Protective Effects of Paeonol, Paeoniliorin and Their Compatibility on In vitro Cultured Cardiomyocytes Suffering from Hypoxia-reoxygenation Injury
摘要
Abstract
Objective To observe the protective effects of paeonol, paeoniflorin, and their compatibility on in vitro cultured cardiomyocytes suffering from hypoxia-reoxygenation injury. Methods Cardiomyocytes from neonatal rats were in vitro cultured and injured by a hypoxia of 2.5 -5 h and a following 2-h reoxygenation. To observe the effects of paeonol and paeoniflorin, four doses of 100,75,50 and 25 mg/L were respectively set up. And to observe the compatibility of paeonol and paeoniflorin, five closes were set up as follows: paeonol 40 and 20 mg/L, paeoniflorin 40 and 20 mg/L, compatibility of paeonol 20 mg/L and paeoniflorin 20 mg/L. The above drugs were incubated with cardkxnyo-cytes during the hypoxia and reoxygenation period respectively. No drug intervention was given to the model group, while no modeling was given to the normal control group. The transudatory creatine kinase (CK), lactate dehydrogen-ase (LDH), and malondialdehyde (MDA) in the culture medium were determined after the hypoxia period and the reoxygenation period respectively, and the total outleakage and the leakage inhibition ratio during the whole procedure were calculated. Results of each group were got from parallel operations for 5 times. Results Compared with the normal control group, the MDA leakage increased 2.5 h after hypoxia, the leakage and the total outleakage of CK, LDH, and MDA all significantly increased 3 and 5 h after hypoxia, and 2 h after reoxygenation. The leakage inhibition ratio of each index decreased with statistical difference (P<0.01, P<0.05). Compared with the model group, the leakage and the total outleakage of LDH and MDA both decreased in the high dose paeonol group, and the high and middle dose pae- oniflorin groups after hypoxia and 2 h after reoxygenation (P<0.01, P<0.05), and the leakage inhibition ratio of each index increased (P<0.01, P<0.05). However, the leakage and the total outleakage of CK decreased in the low dose and the extreme low dose paeonol groups only 2 h after reoxygenation (P<0.01, P<0.05), while the leakage inhibition ratio of CK increased (P <0.01). The leakage and the total outleakage of LDH decreased in the extreme low dose paeoniflorin group only 2 h after reoxygenation (P<0.01), while the leakage inhibition ratio of LDH increased (P< 0.01). The effects of their compatibility showed no significant difference (P >0.05). Conclusions Paeonol, paeoniflorin , and their compatibility all have remarkable protective effects on in vitro cultured cardiomyocytes suffering from hypox-ia-reoxygenation injury. There was no significant synergistic effect when paeonol was used with paeoniflorin together.关键词
丹皮酚/芍药苷/心肌细胞/缺氧/复氧Key words
paeonol/ paeoniflorin/ cardiomyocyte/ hypoxia/reoxygenation引用本文复制引用
张金艳,李澎,李贻奎,赵乐,贾广波..丹皮酚、芍药苷及二者配伍对体外培养心肌细胞缺氧/复氧损伤的影响[J].中国中西医结合杂志,2012,32(4):510-514,5.基金项目
国家自然科学基金青年科学基金项目(No.30801531) （No.30801531）
