中国中西医结合杂志2012，Vol.32Issue(4)：515-520,6.
大鼠心血瘀阻证心肌组织代谢组学的研究
Metabolomics Study of the Myocardial Tissue of Cardiac Blood Stasis Syndrome
摘要
Abstract
Objective To find out the metabolite profile of rats' myocardial tissue of cardiac Wood stasis syndrome (CBSS), and to analyze the metabolic pathway of CBSS rats' myocardial tissue by observing the changes of phynotypes intervened by Yangxin Tongmai Recipe (YTR). Methods Acute myocardial infarction (AMI) rat model of CBSS was prepared by ligating the left anterior descending coronary artery. Meanwhile, the model was interfered with YTR. The metabolites of rats' myocardial tissue were detected in the model group, the YTR group, the sham-operation group, and the blank control group using GC-MS (8 rats in each group). Changes of metabolite contents were analyzed among different groups using principal component analysis (PCA) and least-square analysis. Results As for PCA: The results of PCA showed that principal component integral (PCI) of the four groups was mainly distributed in the three regions of oval scatterplot. The factor loading gram showed that contents of gly-cine, fumaric acid, malic acid, glutamic acid, glucose, phosphoric acid, galactopyranose, lysine were changed in the model group. Analysis of partial least square method; PLS regression model showed that obvious linear correlation existed between the model group and the YTR group, which proved the model was reasonably established. The drug intervention was highly positively correlated with glycine, malic acid, glutamic acid, glucose, highly corre- lated with urea and butanedioic acid, but negatively correlated with lysine. According to VIP value, each variable was closely correlated with the drug intervention in sequence as malic acid, glutamic acid, glycine, glucose, fumar-ic acid, urea, galactose, tyrosine, lactic acid, and alanine. Results of variability analysis; Obvious changed variability analysis of metabolite difference showed that 10 metabolites such as glycine, etc. obviously decreased in the model group, showing significant difference when compared with the normal group (P<0.01). Compared with the model group, contents of glycine, fumaric acid, malic acid, glutamic acid, glucose, tyrosine,urea, lactic acid, and alanine, etc. obviously increased after drug intervention (P <0.01). Of them, the increment of malic acid, glumatic acid, tyrosine, and urea was less, showing significant difference when compared with that of the normal group. The mean of lysine was slightly lowered after drug intervention, but with insignificant difference when compared with that of the model group. AMI rats of CBSS was closely correlated with myocardial metabolites such as malic acid, glutamic acid, glycine, glucose, fumaric acid, urea, galactopyranose, lactic acid, alanine, and tyrosine, etc. Conclusions The metabolite profile of rats' myocardial tissue showed AMI rat model of CBSS was closely correlated with post-hypoxia glucose metabolism disorder. YTR could effectively intervene this process.关键词
代谢组学/心血瘀阻证/心肌组织Key words
metabolomics/ cardiac blood stasis syndrome/myocardial tissue引用本文复制引用
简维雄,陈清华,黄献平,郑景辉,王丽萍,胡志希,孙贵香,袁肇凯..大鼠心血瘀阻证心肌组织代谢组学的研究[J].中国中西医结合杂志,2012,32(4):515-520,6.基金项目
国家自然科学基金资助项目(No.30973717) （No.30973717）
湖南省科技厅资助项目(No.2007FJ3071) （No.2007FJ3071）
湖南省研究生创新基金项目(No.湘财教指[2008]68号) （No.湘财教指[2008]68号）
湖南中医药大学校级青年教师项目(No.48010101-1) （No.48010101-1）
