中国中西医结合杂志2012，Vol.32Issue(4)：537-542,6.
蝎毒多肽提取物促进环磷酰胺抑制Lewis肺癌的作用机制研究
Study on the Mechanism of Polypeptide Extract from Scorpion Venom to Promote the Restraint of Cyclophosphamide on Lewis Lung Cancer
摘要
Abstract
Objective To explore the mechanism of polypeptide extract from scorpion venom (PESV) on promoting anti-tumor effects of cyclophosphamide (CTX). Methods The Lewis lung tumor model was established by subcutaneously implanting Lewis lung cells into C57BL/6 mice. The tumor-bearing mice were randomly divided into 4 groups, i.e., the model group, the cyclophosphamide (CTX) group, the polypeptide extract from scorpion venom (PESV) group, and the combination group (CTX + PESV), 10 mice in each group. The tumor growth curve was recorded. Changes of vascular endothelial growth factor-A (VEGF-A ) and transfroming growth factor-p, (TGF-p,) expressions in the tumor microenvironment were detected using reverse transcription PCR and immunohistochemical assay. Changes of dendritic cells (DCs) phenotype CD80 and CD86 expressions in the tumor tissue were detected using immunofluorescence chemical assay. Results After 21 successive days of treatment, the growth of Lewis lung cancer transplantation tumor in the combination group was obviously inhibited (P<0.05). Compared with the model group,the expressions of CD80 and CD86 in the PESV group was somewhat enhanced, while those in the CTX group was somewhat lowered. Compared with the CTX group, the fluorescent signal strength and expressions in the combination group somewhat increased. Compared with the model group, the expressions of TGF-p, and VEGF-A mRNA decreased in the PESV group and the CTX group (both P<0. 05). Compared with the PESV group and the CTX group, the expressions of TGF-p, and VEGF-A in the combination group both decreased (both P<0.05). Conclusion PESV could inhibit the expressions of VEGF and TGF-p,, promote the maturation of DCs, recover its antigen uptake presentation function, and reverse the immune injury to the body by CTX, thus playing a role in inducing the tumor cell apoptosis.关键词
蝎毒多肽提取物/Lewis肺癌/血管内皮生长因子-A/转化生长因子-β1/树突细胞Key words
polypeptide extract from scorpion venom/ Lewis lung cancer/ vascular endothelial growth fac-tor-A / transfroming growth factor-β1/ dendritic cell引用本文复制引用
宁云娜,张维东,武利存,张月英,贾青,王兆朋,王朝霞..蝎毒多肽提取物促进环磷酰胺抑制Lewis肺癌的作用机制研究[J].中国中西医结合杂志,2012,32(4):537-542,6.基金项目
国家自然科学基金资助项目(No.30873408,No.81073102) （No.30873408,No.81073102）
山东省自然科学基金资助项目(No.2009ZRC0344,No.ZR2009CL030,No.Y2008C176) （No.2009ZRC0344,No.ZR2009CL030,No.Y2008C176）
济南市科学技术发展计划项目(No.201004012) （No.201004012）
