中国中西医结合杂志2012，Vol.32Issue(6)：789-792,4.
补肾壮筋汤含药血清对力学刺激诱导软骨细胞凋亡的影响
Effects of Bushen Zhuangjin Decoction Containing Serum on the Apoptosis of Chondrocytes Induced by Mechanics Stlmulus
摘要
Abstract
Objective To study the effects of Bushen Zhuangjin Decoction (BZD) containing serum on the apoptosis of chondrocytes induced by mechanics stimulus. Methods The BZD containing serum was extracted. The chondrocyte nutritive media was divided into 3 groups, i. e., the common nutritive medium group, the blank rabbit serum medium group, and the BZD nutritive medium group. The apoptosis of chondrocytes was induced by continuing mechanics stimulus in 24 h. Then the chondrocytes were collected. The apoptosis rate of chondrocytes was determined by flow cytometry. The contents of interleukin 1β (IL-1 β) and nitric oxide (NO) in the corresponding media were determined. Results The apoptosis of chondrocytes in the BZD nutritive medium group (19. 55 ±7.98)% was lower than that of the common nutritive medium group (39. 32 ±13.45)% and the blank rabbit serum medium group (37. 87 ±9. 67)%, showing statistical difference (P<0.05). The contents of IL-1 β and NO were also lower in the BZD nutritive medium group with statistical difference when compared with those of the other two groups (P<0.05). Conclusion BZD containing serum could protect mechanics stimulus induced apoptosis of chondrocytes.关键词
补肾壮筋汤/软骨细胞/凋亡/力学/含药血清Key words
Bushen Zhuangjin Decoction/ chondrocyte/ apoptosis/ mechanics/ drug containing serum引用本文复制引用
周国顺,李雄峰,管国华,李建有,黄胜..补肾壮筋汤含药血清对力学刺激诱导软骨细胞凋亡的影响[J].中国中西医结合杂志,2012,32(6):789-792,4.基金项目
浙江省医药卫生科学研究基金计划(No.2008A151) （No.2008A151）
