中药材2012，Vol.35Issue(2)：206-209,4.
云南续断药材HPLC特征指纹图谱研究
Study on HPLC Characteristic Fingerprint of Yunnan Dipsacus asper
摘要
Abstract
Objective: To study the HPLC fingerprint of yunnan Dipsacus asper and provide a reliable method for sciencetific evaluation and quality control. Methods: 16 batches of Dipsacus asper were collected from 11 counties (cities, districts) of 5 prefectures or municipals in Yunnan provinice,which were the mainly distribution region of Dipsacus asper. Samples were analyzed on an Aglient Zorbax SB-18(4.6 mm×250 mm,5 μn) eluted with methanol (A) and water (B) as mobile phase in gradient clution.The flow rate was 1 mL/min,and the column temperature was set at 38℃. The detector wavelength was 220 run. Results; A HPLC fingerprint method was established, 14 common peaks were selected and the similarity ranged from 0. 674 to 0. 965,cluster analysis could classified these 16 batches of Dipsacus asper into 2 groups. Conclusion: Dipsacus japonicus is found for the first time in yunnan. The fingerprint of Dipsacus asper in different origin have a extremely high similarity;The method is accurate and reliable.关键词
续断/HPLC/指纹图谱Key words
Dipsacus asper Wall. ex Henry/HPLC/ Fingerprint分类
医药卫生引用本文复制引用
杨紫刚,许刚,李龙根,孟珍贵,杨生超..云南续断药材HPLC特征指纹图谱研究[J].中药材,2012,35(2):206-209,4.基金项目
云南省科技创新强省计划重点产业创新工程(中药现代化二期)专项(2008IF017) （中药现代化二期）
云南省中青年学术技术带头人后备人才培养计划(2009C1053) （2009C1053）
