中药材2012，Vol.35Issue(2)：254-259,6.
升板方对SD大鼠CYP3A1酶活性的影响
Effect of Shengbanfang on CYP3A1 Activities of Sprague-Dawley Rat
摘要
Abstract
Objective:To study the influences of Shengbanfang on CYP3A1 activities of SD rat and provide suggestions for drug combinations. Methods:25 male SD rats were devided into 5 groups randomly,and treated with saline(NS, ig,10mg/(kg/d) ,qd,14 d),dexamethasone(DEX,ig,100mg/(kg·d),qd,3 d),high dose of Shengbanfang ( HD, ig, 8.645g/kg, bid, 14 d), middle dose of Shengbanfang (MD,ig,4. 322g/kg,bid,14 d) and low dose of Shengbanfang(LD,ig,2.161g/kg,bid,14 d),respectively. The HPLC method was established and validated to determine the productive velocity of 6β-hydroxytestosterone and measure the activity of CYP3A1. Results:Under the optimized incubation conditions,the productive rates of 6β-hydroxytestosterone of HD,MD,LD,NS and DEX, groups were (55. 82 ±5. 97), (65. 10 ±6. 83) , (60.89 ±6. 53) , (62.17 ±6.55), (126.73 ± 15.40) μmol/( L·mg pro·min). There were significant differences between Shengbanfang groups compared with dexamethasone group,but there was no significant difference between Shengbanfang groups and the control group(NS). Conclusion: Shengbanfang has no induce effect on the enzymic activity of CYP3A1 in SD rats.关键词
升板方/睾酮/6β-羟基睾酮/肝微粒体/CYP3A1/HPLCKey words
Shengbanfang/Testosterone/6β-hydroxytestosterone/Liver microsomes/CYP3Al /HPLC分类
医药卫生引用本文复制引用
刘韬,邓多,林子超,潘莹,周文菁,周望,黄红兵..升板方对SD大鼠CYP3A1酶活性的影响[J].中药材,2012,35(2):254-259,6.基金项目
广东省中医药管理局建设中医药强省科研基金项目(2009167) （2009167）
广东省医学科研基金项目(B2009090) （B2009090）
