中药材2012，Vol.35Issue(2)：264-269,6.
葡萄籽原花青素增强X射线辐射敏感性的实验研究
Study on the Effect of Grape Seed Proanthocyanidins on Increasing the Roadiosensitivity for X-ray
摘要
Abstract
Objective:To investigate the effect of Grape Seed Proanthocyanidins ( GSPs) on enhancing the radiosensitivity of human hepatic carcinoma cell line HepG2,human cervical cancer cell line Hela and human leukemia cell line K562 for X-ray in vitro: Methods:The killing effect of GSPs combined with X-ray on cells was evaluated by SRB and clone formation assay. Results:The GSPs had obvious cytotoxicity on three cell lines in a dose-dependent and time-dependent manners. However,inhibition rate of different cell line were quite different,the strongest one was human leukemia K562 cells and the others were weak. The sensitization ranges calculated by univariate analysis were 6. 25 ~ 12.5μg/mL for human leukemia K562 cells. Sensitization enhancement ratio was 1. 94 using curve fining method for K562 cells. Conclusion:GSPs can obviously enhance the radiosensitivity of cancer cells in vitro. The mechanism of sensitization effect may be related to the effects of GSPs on oxygen balance and cell cycle.关键词
葡萄籽原花青素/肝癌细胞株/宫颈癌细胞株/白血病细胞株/X射线/增敏Key words
GSPs /Hepatoma carcinoma cell HepG2/ Human cervical cancer cell line Hela / Human leukemia cell line K562/X-rav/Sensitizatinn分类
医药卫生引用本文复制引用
潘晓婧,王敏,王学习,刘斌,张红..葡萄籽原花青素增强X射线辐射敏感性的实验研究[J].中药材,2012,35(2):264-269,6.基金项目
甘肃省自然科学基金项目(0710RJZA016) （0710RJZA016）
兰州大学交叉学科青年创新基金(LZUJC200917) （LZUJC200917）
教育部科研基本费小额探索项目(Izujbky-2011-134) （Izujbky-2011-134）
