中药材2012，Vol.35Issue(2)：304-306,3.
杜仲叶桃叶珊瑚苷的酶法提取及其抑菌活性
Enzymatic Extraction and Antibacterial Activity of Aucubin from Eucommia ulmoides Leaves
郑杰 1刘端 1赵肃清 1苏娟 1颜秋萍 1陈磊 1肖奕 1张春梅1
作者信息
- 1. 广东工业大学轻工化工学院,广东广州510006
- 折叠
摘要
Abstract
Objective: To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity. Methods: Aucubin in Eucommia ulmoides leaves was extracted by cellulase method. The extraction technology was optimized using the content of Aucubin as index. The antibacterial activity of Aucubin was determined. Results:The results showed that the optimum technology was as follows; The solid-liquid ratio was 1:12, extracted for 50 min by 0.4% enzyme at 50 ℃ in pH 6.0. The extraction rate of Aucubin was as high as 17. 892 mg/g. The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus, the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively. However, Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus, the MIC of Aucubin against them were all 28.946 mg/mL. Conclusion:The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate, and the Aucubin has some antibacterial activity.关键词
杜仲叶/酶解提取/桃叶珊瑚苷/抑菌作用Key words
Eucommia ulmoides leaves/ Enzymatic hydrolysis extraction/ Aucubin/ Antibacterial effect分类
医药卫生引用本文复制引用
郑杰,刘端,赵肃清,苏娟,颜秋萍,陈磊,肖奕,张春梅..杜仲叶桃叶珊瑚苷的酶法提取及其抑菌活性[J].中药材,2012,35(2):304-306,3.
