中药材2012，Vol.35Issue(2)：306-310,5.
大孔树脂纯化绵茵陈中总有机酸和绿原酸的研究
Study on Purification of Total Organic Acld and Chlorogenic Acid in Herba Artemisiae Scopariae with Macroporous Adsorption Resin
孙宗喜 1徐桂花 2李艳芳 1吕晓慧1
作者信息
- 1. 鲁南制药集团股份有限公司,山东临沂276005
- 2. 中药制药共性技术国家重点实验室,山东临沂276005
- 折叠
摘要
Abstract
Objective:To optimize the technological parameters of the purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with macroporous adsorption resin. Methods: Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin. Then orthogonal design L9 (34) and single factor experiment were used to selecl the optimum purification process conditions. Results: The optimal purification process for total organic acid and chlorogenic acid with
HPD200A maeroporous adsorption resin were as follows:the diameter height ratio of the resin was 1:6, the sample concentration was 1 g/mL, the absorption velocity was 1 BV/h (1 BV represented one column volume), the ratio of sample to HPD200A macroporous adsorption resin was 1.5:1 ( W/W), 3 BV of water was used as purificant and 2 BV of 90% ethanol was used as eluant Under these conditions, the purity of total organic acid and chlorogenic acid was 588.74% and 567.89% , respectively. Conclusion:The established purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with HPD200A macroporous adsorption resin is simple and stable and can be used in industrial production.关键词
大孔树脂/纯化/绵茵陈/总有机酸/绿原酸Key words
Macroporous adsorption resin/ Purity/ Herba Artemisiae scopariae/ Total organic acid/ Chlorogenic acid分类
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孙宗喜,徐桂花,李艳芳,吕晓慧..大孔树脂纯化绵茵陈中总有机酸和绿原酸的研究[J].中药材,2012,35(2):306-310,5.
