组织工程与重建外科杂志2012，Vol.8Issue(2)：69-72,4.DOI:10.3969/j.issn.1673-0364.2012.02.003
角质形成细胞PAR-2基因siRNA的筛选
Screening of siRNA Targeted PAR-2 in Keratinocyte
摘要
Abstract
Objective To design and screen small interfering RNA (siRNA) targeted prolease-activated receptor-2 (PAR-2) mRNA in Keratinocyte that can specifically and effectively suppress PAR-2 expression. Methods Three pairs of PAR-2 specific double stranded siRNAs were designed according to the sequence of PAR-2 mRNA. These siRNA were tranafected into human keratinocyte by Iipofectamine 2000. The transcription level of PAR-2 was analyzed by semi-quantitative RT-PCR and PAR-2 protein expression was tested by western blot in order to evaluate the effect of RNA interference. Results Two of the three PAR-2 siRNA effectively inhibited the expression of PAR-2 and the second one is more effective. Conclusion PAR -2 siRNAs can be successfully designed and synthesized,which can specifically and effectively suppresses the expression of PAR-2 gene. The results in this study lay the foundation for further study on PAR-2 gene silencing by RNA interfere (RNAi) .关键词
角质形成细胞/蛋白酶激活受体-2/RNA于扰Key words
Keratinocyte/ Protease-activated receptor-2/ RNA interfere分类
生物科学引用本文复制引用
袁磊,金蓉,张艳,张余光..角质形成细胞PAR-2基因siRNA的筛选[J].组织工程与重建外科杂志,2012,8(2):69-72,4.基金项目
上海市教育委员会科研项目(06BZ021) （06BZ021）
