组织工程与重建外科杂志2012，Vol.8Issue(2)：73-77,5.DOI:10.3969/j.issn.1673-0364.2012.02.004
丹参酮ⅡA诱导瘢痕疙瘩成纤维细胞凋亡及细胞周期阻滞的实验研究
Effect of Tanshinone ⅡA on Cell Proliferation, Apoptosis and Cell Cycle of Fibroblasts Derived from Keloid
陈刚 1梁奕敏 2李青峰2
作者信息
- 1. 510280 广东省广州市 南方医科大学附属珠江医院整形外科
- 2. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 折叠
摘要
Abstract
Objective To explore the effect of Tanshinone E A on cell proliferation, apoptosis and cell cycle of Fibroblasts derived from keloid. Methods Fibroblasts derived from keloid were cultured with different concentration of Tanshinone IIA (0 p-g/mL, 50 jig/mL, 100 (Xg/mL and 200 u.g/mL) in vitro. CCK-8 was used to detect cell proliferation, and flow cytometry was used to analyze cell apoptosis, cell cycle at the different time. Results Cell proliferation of fibroblasts derived from keloid was decreased, apoptosis at the early phase was increased, and cell cycle in G0/G1 phase was arrested by different concentration of Tanshinone HA with different intervention lime (P<0.001), especially in 200 |Xg/mL group. Variance analysis showed that those differences have statistical significance. Conclusion Tanshinon II A can suppress cell proliferation, induce cell apoptosis and arrest cell cycle of fibroblasts derived from keloid. The effect of Tanshinone IIA was affected by different concentration and different intervention time.关键词
瘫痕疙瘩/成纤维细胞/丹参酮ⅡA/细胞凋亡/细胞周期Key words
Keloid/ Fibroblast/ Tanshinone HA/ Apoptosis/ Cell cycle分类
生物科学引用本文复制引用
陈刚,梁奕敏,李青峰..丹参酮ⅡA诱导瘢痕疙瘩成纤维细胞凋亡及细胞周期阻滞的实验研究[J].组织工程与重建外科杂志,2012,8(2):73-77,5.
