组织工程与重建外科杂志2012，Vol.8Issue(2)：78-83,6.DOI:10.3969/j.issn.1673-0364.2012.02.005
软骨形态发生蛋白1诱导瘢痕成纤维细胞表达软骨表型的实验研究
Cartilage-Derived Morphogenetic Protein 1 in the Induction of Chondrogenic Differentiation of Scar Fibroblasts in Vitro
马晓飞 1张艳 1柴岗 1朱明1
作者信息
- 1. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 折叠
摘要
Abstract
Objective To investigate the feasibility of the differentiation of scar fibroblasts into chondrogenic phenotype in vitro induced by Cartilage-derived morphogenetic protein 1 (CDMP1) for the reconstruction of ear defect. Methods Scar fibroblasts were extracted from hypertrophic scar and cultured in momo -layer in vitro. Scar fibroblasts of the 4th passage were induced by CDMP1 (100 ng/mL in medium of F12+2%FBS). Negative and positive control groups were also set up. After 7 days of induction, morphological change of cells was observed by Image Plus software. Expression of col I , col H , sox 9 and aggrecan were detected by inununofluorescence, RT-PCR and western blot. Micromass cultured scar fibroblasts were induced and cultured. The results were analyzed by HE staining, immunohistocheraistry and Safranine-0 staining. Results After induction, the morphology of the cells changed from spindle to polygon shape. By Image Plus software analysis, the length/width ratio changed from (7.21?.54):1 of pre-induction to (1.48?.21):l of post-induction (P<0.05). No significant difference was found between post-induction fibroblasts and normal chondrocytes (P>0.05). Col II, aox9, aggrecan were expressed in post-inductive cells by immunofluorescence, RT-PCR and western blot. HE staining, immunohistochemistry and Safranine-0 staining revealed cartilage specific structure and deposition of cartilage extracellular matrix in micromass cultured scar fibroblasts. Conclusion Scar fibroblasts could be differentiated into chondrogenic phenotype by CDMP1 induction and induced scar fibroblasts can be used as the seed cells for the reconstruction of ear defect.关键词
瘢痕成纤维细胞/软骨细胞/软骨形态发生蛋白1分化Key words
Scar fibroblasts/ Chondrocytes/ CDMP1/ Differentiation分类
生物科学引用本文复制引用
马晓飞,张艳,柴岗,朱明..软骨形态发生蛋白1诱导瘢痕成纤维细胞表达软骨表型的实验研究[J].组织工程与重建外科杂志,2012,8(2):78-83,6.
