安徽工程大学学报2012，Vol.27Issue(2)：1-3,7,4.
黑曲霉糖化酶基因在酿酒酵母中的表达
Cloning and expression of glucoamylase genes from aspergilus niger cDNA library in S. cerevisiae
钱鹏 1汤斌1
作者信息
- 1. 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖241000
- 折叠
摘要
Abstract
An expression cDNA library was constructed from aspergillus niger, then glucoamylase gene was isolated and efficiently expressed in S. cerevisiae. The results showed that the maximum activity of glucoamylase was 4.2 U/mL when cultivated for 60 h. Sequence analysis revealed that glucoamylase had 1 908 bp,which encodes a putative polypeptide of 636 amino acids. The enzyme proerties showed that the optimum pH and temperature was 5.0 and 50 ℃, the expressed protein was purified from the fermented supernatant using DEAE clumon and determined by SDS-PAGE. The results of SDS-PAGE also showed that the molecular weights of the enzyme was 70 kDa.关键词
黑曲霉/糖化酶/酿酒酵母/表达Key words
aspergilus niger/glucoamylase/S. cerevisiae/expression分类
生物科学引用本文复制引用
钱鹏,汤斌..黑曲霉糖化酶基因在酿酒酵母中的表达[J].安徽工程大学学报,2012,27(2):1-3,7,4.
