安徽工程大学学报2012，Vol.27Issue(2)：27-30,4.
姜黄素钐配合物与牛血清白蛋白相互作用研究
On the interaction of curcumin-samarium coordination and bovine serum albumin
方瑞萍 1陈宁生 1施金才1
作者信息
- 1. 安徽工程大学生物与化学工程学院,安徽芜湖241000
- 折叠
摘要
Abstract
The binding of curcumin-samarium coordination and bovine serum albumin（BSA） was investi- gated by fluorescence method. According to the fluorescence quenching effect of the complex-BSA, the variable-temperature experiments were finished, from which the binding constants, the number of binding sites and the values of thermodynamic parameters were obtained. The quenching mechanism and the type of binding force were inferred. With the theory of forster energy transfer the distance was calculated. The probe was used for positioning and the synchronous fluorescence spectroscopies were scanned to monitor the effect of the complex on BSA. The results showed that the complex quenched the intrinsic fluorescence of BSA mainly by a static process;meanwhile the non-radiative energy transfer occurred. △Hθ and △sθ〈0 , suggesting van der Waals forces and hydrogen bonding was the main binding force. The binding occurred in the Site II of BSA molecular and the distance was 2.4 nm. The conformation of BSA molecu- lar was modified by the complex,which had a greater effect on tryptophan residues.关键词
姜黄素/钐/牛血清白蛋白/荧光/紫外可见光谱Key words
curcumin/samarium/bovine serum albumin/fluorescence/UV-visible spectroscopy分类
化学化工引用本文复制引用
方瑞萍,陈宁生,施金才..姜黄素钐配合物与牛血清白蛋白相互作用研究[J].安徽工程大学学报,2012,27(2):27-30,4.
