现代农业科技Issue(8)：318-320,3.
基于ITS-1序列的部分鳚亚目鱼类的分子系统进化关系研究
Research on Molecular Phylogenetic Relationship of Partial Blennioidei Based on ITS-1 Sequences
摘要
Abstract
In this study,the internal transcribed spacer 1（ITS-1）partial sequences of 3 species from Blennioidei in China yellow and bohai seas were amplified using PCR technique,Scomberomorus regalis was designed as outgroup.Nucleotide composition frequencies,Kimura-2 parameter genetic distance and transition/transversion ratios were analyzed with MEGA 4.0 software.Molecular phylogenetic trees were constructed by maximum parsimony（MP）and neighborjoining（NJ）methods also with MEGA 4.0.The results were as follows：There were insertions and deletions in the aligned base pairs,49 bp variable sites were found in the analysis matrix.,the average ts/tv ratio was 1.0;The length of ITS1 was from 375 bp to 562 bp,with the Enedrias fangi′s the longest,and the Ernogrammus hexagrammus the shortest.Enedrias nebulosus and Enedrias fangi were placed in a same clade with support of a high bootstrap value.Enedrias fangi and Enedrias nebulosus had a close relationship,as the genetic distance between the two species of ITS-1 was only 0.02.关键词
鳚亚目/线粒体DNA/ITS-1基因序列/系统发育Key words
Blennioidei/mitochondrial DNA(mtDNA)/internal transcribed spacer 1(ITS-1)/molecular phylogeny分类
生物科学引用本文复制引用
张源真,王伟,姜志强..基于ITS-1序列的部分鳚亚目鱼类的分子系统进化关系研究[J].现代农业科技,2012,(8):318-320,3.基金项目
辽宁省重大科技攻关项目 （）
辽宁省教育厅计划 （）
