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金钗石斛的离体快繁体系研究

罗林会 王勤

安徽农业科学2012,Vol.40Issue(15):8476,8479,2.
安徽农业科学2012,Vol.40Issue(15):8476,8479,2.

金钗石斛的离体快繁体系研究

Study on the in vitro Rapid Propagation System of Dendrobium nobile Lindl

罗林会 1王勤1

作者信息

  • 1. 遵义职业技术学院,贵州遵义563006
  • 折叠

摘要

Abstract

[ Objective] To study the in vitro rapid propagation system of D. nobile. f Method] The seeds of D. nobile were selected for the in vitro rapid propagation by tissue culture. [ Result] MS + I mg/L BA + 0.02 mg/L N AA + 2% potato juice + 3 % sucrose was suitable for the protocorm induction and hud differentiation of D. nobile. The inductive rate was 7 - 10. The most suitable rooting medium was 1/2 MS + 0. 2 mg/L BA + 0.2 mg/L NAA + 0.2 mg/L GA + 2% banana juice + 2% potato juice + 2% sucrose. The rooting rate was above 98% , and survival rate was above 90%. [ Conclusion ] The method studied the in vitro rapid propagation system of D. nobile and provided references for the tissue culture of its seedlings.

关键词

金钗石斛(Dendrobium nobile Lindl.)/离体快繁/培养基

Key words

Dendrobium nobile Lindl. / In vitro rapid propagation/ Culture medium

分类

农业科技

引用本文复制引用

罗林会,王勤..金钗石斛的离体快繁体系研究[J].安徽农业科学,2012,40(15):8476,8479,2.

安徽农业科学

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