安徽农业科学2012，Vol.40Issue(15)：8731-8735,8766,6.
响应面法优化酶解提取金枪鱼鱼油的工艺研究
Optimization of the Enzymatic Extraction of Tuna Oil by Response Surface Methodology
摘要
Abstract
[ Objective ] The aim was to study the extraction technology of fish oil by protease, so as to provide references for its study. [ Method] The mixed solution of tuna oil and water was hydrolyzed with trypsin. With the extraction rate offish oil as the investigation target, the response surface methodology was adopted to optimize the extraction technology of tuna oil. [Result] The best extraction conditions were; 1. 71% of trypsin dose, 7.94 of pH, 4. 22 h of extraction time and 44 ? of extraction temperature, under these conditions, the extraction rate offish oil could be up to 90.23% , the degummed, deacidified, decolored and deodorized fish oil met the first-grade standard, its DHA and EPA were 27.71% and 5.94% , respectively. [Conclusion] It is practical to extract the fish oil from the mixed tuna oil and water solution through the hydrolysis of trypsin.关键词
酶解/蛋白酶/响应面法/鱼油Key words
Enzymolysis/Protease/ Response surface methodology/ Fish oil分类
农业科技引用本文复制引用
何键东,王朋,胡烨,罗红宇..响应面法优化酶解提取金枪鱼鱼油的工艺研究[J].安徽农业科学,2012,40(15):8731-8735,8766,6.基金项目
浙江省社会发展重大科技项目(2009C03017-2) （2009C03017-2）
2009年浙江省大学生项目科技创新活动计划(新苗人才计划). （新苗人才计划）
